1. Identification of a functional domain in MKL1_S that allows specific transcriptional activity.
Identification of a functional domain in MKL1_S that allows specific transcriptional activity. (A) Alignment of N-terminal amino acid sequences of the MKL1 isoforms. Green box, predicted 9aa TAD in MKL1_S; green letter, published ATG/Met start of MKL1_L; red letter, ATG/Met start of MKL1_S. (B) Schematic representations of the constructs used. A cross indicates a sequence that does not fulfill the 9aa TAD criteria. (C) qPCR analysis of MKL1_S-specific target genes. Constructs indicated were stably overexpressed in HEK293 cells. Before RNA extraction, cells were starved and MKL1 activity stimulated with LPA. MKL1_L and MKL1_S, 5′UTR-full-length constructs; ΔC constructs, MKL1 missing its C-terminal half. Data are represented as mean±s.d. of two independent experiments each with two independently created overexpressing cell lines (n = 4). ***P<0.001 [MMP16 levels; one-way ANOVA (Holm–Sidak method)].
Matthias A. Scharenberg et al. J Cell Sci 2014;127:
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