1. Intracellular cytokine expression of peripheral blood natural killer cell subsets in women with recurrent spontaneous abortions and implantation failures 
Atsushi Fukui, M.D., Joanne Kwak-Kim, M.D., Evangelos Ntrivalas, M.D., Alice Gilman-Sachs, Ph.D., Sung-Ki Lee, M.D., Kenneth Beaman, Ph.D. 
Fertility and Sterility 
Volume 89, Issue 1, Pages (January 2008)
DOI: /j.fertnstert
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Representative dot plots of cytokine production by peripheral blood CD56+ cells. We classified results of flow cytometric analysis as follows. (A, B) R1 = CD56−/cytokine+ cells; R2 = CD56dim/cytokine+ cells; R3 = CD56bright/cytokine+ cells; R4 = CD56−/cytokine− cells; R5 = CD56dim/cytokine− cells; R6 = CD56bright/cytokine− cells. CD56+ cells were double stained for TNF-α and IFN-γ intracellular cytokine expression: IFN-γ+/TNF-α (R7), IFN-γ+/TNF-α+ (R8), and IFN-γ−/TNF-α+ (R10). Natural killer-1 cells are defined as IFN-γ– or TNF-α–producing NK cells, the sum of R7, R8, and R10 (C). Natural killer-2 cells are IL-4–, IL-5–, or IL-13–producing NK cells. CD56+ cells were double stained for IL-4 and IL-10 intracellular cytokine expression: IL-10+/IL-4− (R7), IL-10+/IL-4+ (R8), and IL-10−/IL-4+ (R10) (D).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Tumor necrosis factor-α–producing CD56bright cells and subsets in women with recurrent SAB (RSA), implantation failure, or controls. Box and whisker plots: bar (horizontal line) = median; box = 25th and 75th percentiles; whiskers = extend to the extreme values. Differences between the three groups were analyzed by Kruskal-Wallis analysis of ranks and are reported on the graph. a = P<.05 and b = P<.01 when compared with controls, analyzed by Mann-Whitney U test.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Tumor necrosis factor-α and GM-CSF ratio in NK cell subsets in women with recurrent SAB (RSA), implantation failure, or controls. Box and whisker plots: bar (horizontal line) = median; box = 25th and 75th percentiles; whiskers = extend to the extreme values. Difference between the three groups was analyzed by Kruskal-Wallis analysis of ranks and are reported on the graph. a = P<.05 and b = P<.01 when compared with controls, analyzed by Mann-Whitney U test.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions