1. Using a Pipette Filler Do NOT let the water get into the bulb
Squeeze Valve A and bulb at the same time
Put pipette in liquid then squeeze Valve S until the liquid is at the 0 mark
Put pipette over new container then squeeze Valve E until the liquid is at the mark for how much you need to dispense
Practice measuring out 4 mL
Safety Pipet Filler This bulb-type safety pipet filler is designed for efficient one-hand operation. Made from natural rubber for better memory and resistance to chemical attack. Its three chemically resistant valves control the evacuation of air, liquid uptake and liquid dispensing. Expel air from the bulb by squeezing the “A” valve and the bulb at the same time. Draw liquid into the pipet by squeezing the “S” valve. To dispense liquid squeeze the “E” valve. To deliver last drop squeeze the small bulb next to the “E” valve.

2. Part 2—How to Prepare a Serial Dilution
Place 4 mL of the 100% blue food coloring into Tube 1 and SET ASIDE. Do not use this tube again for the rest of the activity.
Next, place 4mL of the 100% blue food coloring into Tube 2.
Add 4 mL of water to Tube 2. (Tube 2 now has 8 mL total)
Place your thumb over top of tube and shake gently
Tube 1
Tube 2
SET ASIDE;
NEVER REMOVE FOOD DYE FROM THIS TUBE!
4 mL water
Part 2—How to Prepare a Serial Dilution
Mix
gently
4 mL blue food dye
4 mL blue food dye

3. Part 2—How to Prepare Serial Dilution
Measure out exactly 4 mL of the solution from Tube 2 and put it in to Tube 3. (Tube 2 is back to being just 4 mL)
Add 4 mL of water to Tube 3 and mix as before
Continue this same process for Tubes 4-6.
Tube 6 will have 8 mL of solution in it, simple measure out 4 mL of its contents and discard.
Tube 2
Tube 3
Move 4 mL of
Tube 2 to Tube 3
4 mL water
Part 2—How to Prepare Serial Dilution
8 mL of food dye
and water mixed
Mix
gently
4 mL of Tube 2’s mixed contents
And so on….