Presentation is loading. Please wait.

Presentation is loading. Please wait.

The Modulation of Aromatase and Estrogen Receptor Alpha in Cultured Human Dermal Papilla Cells by Dexamethasone: A Novel Mechanism for Selective Action.

Published bySurya Sudirman Modified over 5 years ago

Similar presentations

Presentation on theme: "The Modulation of Aromatase and Estrogen Receptor Alpha in Cultured Human Dermal Papilla Cells by Dexamethasone: A Novel Mechanism for Selective Action."— Presentation transcript:

1 The Modulation of Aromatase and Estrogen Receptor Alpha in Cultured Human Dermal Papilla Cells by Dexamethasone: A Novel Mechanism for Selective Action of Estrogen via Estrogen Receptor Beta?  M. Julie Thornton, Louisa D. Nelson, Anthony H. Taylor, M. Pattie Birch, Ian Laing, Andrew G. Messenger  Journal of Investigative Dermatology  Volume 126, Issue 9, Pages (September 2006) DOI: /sj.jid Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Human dermal papilla cells express mRNA for ERα, ERβ, and aromatase in culture. Ethidium bromide-stained RT-PCR gels showing the expression of ERα, ERβ, aromatase, and GAPDH mRNA from cultured dermal papilla cells derived from five different patients: Numbers 1–5 denote the five different dermal papilla cell cultures, lane 6 (+) is a human myometrium sample that acted as a positive control for ERα and GAPDH or a human ovary sample that acted as a positive control for ERβ and aromatase. The final lane is a RT control for the assay. Size markers for the 100bp DNA marker (L) are shown to the left of the figure. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 ERα mRNA expression in cultured human dermal papilla cells is reduced by dexamethasone, but not by androgens and estrogens. (a) Ethidium bromide-stained RT-PCR gels showing changes in the levels of ERα mRNA levels in untreated cultured human dermal papilla cell lines (Ctrl, lane a) or when treated for 24hours with either, 10nm estrone (E1, lane b), 10nm 17β-estradiol (E2, lane c), 100nm tamoxifen (Tam, lane d) a combination of 100nm tamoxifen, and 10nm E2 (Tam/E2, lane e), 10nm testosterone (T, lane f), 10nm 5α-DHT (lane g), 10nm 5α-androstane-3α, 17β-diol (Diol 1, lane h), 10nm 5α-androstane-3β, 17β-diol (Diol 2, lane i), or 100nm dexamethasone (Dex, lane j). Numbers to the right indicate the individual cell lines. (b) Graphical depiction of grouped data from (a) after correction for individual GAPDH levels (data not shown). Messenger RNA levels are presented as the mean±SEM and analyzed against the untreated controls. (*P<0.05; Student's t-test with Welch's correction; n=5). Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Androgens, estrogens, and glucocorticoids have no effect on the expression of ERβ mRNA in cultured human dermal papilla cells. (a) Ethidium bromide-stained RT-PCR gels showing changes in the levels of ERβ mRNA levels in untreated cultured human dermal papilla cell lines (Ctrl, lane a) or when treated for 24hours with either, 10nm estrone (E1, lane b), 10nm 17β-estradiol (E2, lane c), 100nm tamoxifen (Tam, lane d) a combination of 100nm tamoxifen and 10nm E2 (Tam/E2, lane e), 10nm testosterone (T, lane f), 10nm 5α-DHT (lane g), 10nm 5α-androstane-3α, 17β-diol (Diol 1, lane h), 10nm 5α-androstane-3β, 17β-diol (Diol 2, lane i), or 100nm dexamethasone (Dex, lane j). Numbers to the right indicate the individual cell lines. (b) Graphical depiction of grouped data from (a) after correction for individual GAPDH levels. Messenger RNA levels are presented as the mean±SEM and analyzed against the untreated controls. (Data are not significantly different; Student's t-test with Welch's correction; n=5). Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Dexamethasone upregulates the expression of aromatase mRNA in cultured human dermal papilla cells, but androgens and estrogens have no effect. (a) Ethidium bromide-stained RT-PCR gels showing changes in the levels of aromatase mRNA levels in untreated cultured human dermal papilla cell lines (Ctrl, lane a) or when treated for 24hours with either, 10nm estrone (E1, lane b), 10nm 17β-estradiol (E2, lane c), 100nm tamoxifen (Tam, lane d) a combination of 100nm tamoxifen and 10nm E2 (Tam/E2, lane e), 10nm testosterone (T, lane f), 10nm 5α-DHT (lane g), 10nm 5α-androstane-3α, 17β-diol (Diol 1, lane h), 10nm 5α-androstane-3β, 17β-diol (Diol 2, lane i), or 100nm dexamethasone (Dex, lane j). Numbers to the right indicate the individual cell lines. (b) Graphical depiction of grouped data from (a) after correction for individual GAPDH levels (data not shown). Messenger RNA levels are presented as the mean±SEM and analyzed against the untreated controls. (*P<0.05; Student's t-test with Welch's correction; n=5). Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Human dermal papilla cells express ERα and ERβ protein in culture. Immunohistochemical localization of ERα and ERβ in cultured dermal papilla cells derived from human non-balding scalp hair follicles. Bound antibody was visualized with diaminobenzadine. (a) Negative control, where the primary antibody was omitted. (b) Expression of ERα seen distributed throughout the cytoplasm. (c) Expression of ERβ, which was confined specifically to the nucleus. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 The active androgens are testosterone and 5α-DHT. Both bind to the androgen receptor with a high affinity and the active estrogen is 17β-estradiol, which binds to both estrogen receptors with similar affinity. However, humans also secrete large amounts of the precursor steroids dihydroepiandrosterone sulfate and androstenedione from the adrenal cortex, which can be converted to the more potent androgens and estrogens by the appropriate enzymes. DHT may be further metabolized by 3β-hydroxysteroid dehydrogenase to 5α-androstane-3β, 17β-diol, which can interact with both ERα and ERβ, or by 3α-hydroxysteroid dehydrogenase to its isomer 5α-androstane-3α, 17β-diol which has weak androgenic effects. A number of different 17β-hydroxysteroid dehydrogenases regulate different oxidative and reductive pathways. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "The Modulation of Aromatase and Estrogen Receptor Alpha in Cultured Human Dermal Papilla Cells by Dexamethasone: A Novel Mechanism for Selective Action."

Similar presentations

Figure 5. 1,25-(OH)2D plus Dex dose response for increased mMCSF by RT-PCR. Semiquantitative RT-PCR for mMCSF and GAP was performed in controls.

Figure 5. 1,25-(OH)2D plus Dex dose response for increased mMCSF by RT-PCR. Semiquantitative RT-PCR for mMCSF and GAP was performed in controls.
IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,
Neurotrophins and Their Receptors Stimulate Melanoma Cell Proliferation and Migration  Francesca Truzzi, Alessandra Marconi, Roberta Lotti, Katiuscia.

Neurotrophins and Their Receptors Stimulate Melanoma Cell Proliferation and Migration  Francesca Truzzi, Alessandra Marconi, Roberta Lotti, Katiuscia.
Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal.

Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal.
Staphylococcus aureus Stimulates Neutrophil Targeting Chemokine Expression in Keratinocytes through an Autocrine IL-1α Signaling Loop  Florina Olaru,

Staphylococcus aureus Stimulates Neutrophil Targeting Chemokine Expression in Keratinocytes through an Autocrine IL-1α Signaling Loop  Florina Olaru,
Laura Campbell, Elaine Emmerson, Helen Williams, Charis R

Laura Campbell, Elaine Emmerson, Helen Williams, Charis R
NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes  Dr C. Lianxu, Ph.D.,

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes  Dr C. Lianxu, Ph.D.,
The Distribution of Estrogen Receptor β Is Distinct to That of Estrogen Receptor α and the Androgen Receptor in Human Skin and the Pilosebaceous Unit 

The Distribution of Estrogen Receptor β Is Distinct to That of Estrogen Receptor α and the Androgen Receptor in Human Skin and the Pilosebaceous Unit 
IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis  Rahul Purwar, Thomas Werfel, Miriam.

IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis  Rahul Purwar, Thomas Werfel, Miriam.
Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes  Marcel B.M. Teunissen, Jan.

Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes  Marcel B.M. Teunissen, Jan.
Elevated sex steroid hormones in great saphenous veins in men

Elevated sex steroid hormones in great saphenous veins in men
Differences in Expression of Specific Biomarkers Distinguish Human Beard from Scalp Dermal Papilla Cells  Susan E. Rutberg, Meredith L. Kolpak, John A.

Differences in Expression of Specific Biomarkers Distinguish Human Beard from Scalp Dermal Papilla Cells  Susan E. Rutberg, Meredith L. Kolpak, John A.
Pseudomonas Aeruginosa- and IL-1β-Mediated Induction of Human β-Defensin-2 in Keratinocytes Is Controlled by NF-κB and AP-1  Kai Wehkamp, Lars Schwichtenberg,

Pseudomonas Aeruginosa- and IL-1β-Mediated Induction of Human β-Defensin-2 in Keratinocytes Is Controlled by NF-κB and AP-1  Kai Wehkamp, Lars Schwichtenberg,
Minoxidil-Induced Hair Growth is Mediated by Adenosine in Cultured Dermal Papilla Cells: Possible Involvement of Sulfonylurea Receptor 2B as a Target.

Minoxidil-Induced Hair Growth is Mediated by Adenosine in Cultured Dermal Papilla Cells: Possible Involvement of Sulfonylurea Receptor 2B as a Target.
Histamine Contributes to Tissue Remodeling via Periostin Expression

Histamine Contributes to Tissue Remodeling via Periostin Expression
Androgen Receptor Co-Activator Hic-5/ARA55 as a Molecular Regulator of Androgen Sensitivity in Dermal Papilla Cells of Human Hair Follicles  Shigeki Inui,

Androgen Receptor Co-Activator Hic-5/ARA55 as a Molecular Regulator of Androgen Sensitivity in Dermal Papilla Cells of Human Hair Follicles  Shigeki Inui,
The mRNA for Protease Nexin-1 is Expressed in Human Dermal Papilla Cells and its Level is Affected by Androgen  Tadashige Sonoda, Yuji Asada, Sotaro Kurata,

The mRNA for Protease Nexin-1 is Expressed in Human Dermal Papilla Cells and its Level is Affected by Androgen  Tadashige Sonoda, Yuji Asada, Sotaro Kurata,
Substance P Stimulates Endothelin 1 Secretion via Endothelin-Converting Enzyme 1 and Promotes Melanogenesis in Human Melanocytes  Phil June Park, Tae.

Substance P Stimulates Endothelin 1 Secretion via Endothelin-Converting Enzyme 1 and Promotes Melanogenesis in Human Melanocytes  Phil June Park, Tae.
Keratinocyte-Releasable Stratifin Functions as a Potent Collagenase-Stimulating Factor in Fibroblasts  Aziz Ghahary, Feridoun Karimi-Busheri, Yvonne Marcoux,

Keratinocyte-Releasable Stratifin Functions as a Potent Collagenase-Stimulating Factor in Fibroblasts  Aziz Ghahary, Feridoun Karimi-Busheri, Yvonne Marcoux,
Active Influx Transport is Mediated by Members of the Organic Anion Transporting Polypeptide Family in Human Epidermal Keratinocytes  Ruth Schiffer, Mark.

Active Influx Transport is Mediated by Members of the Organic Anion Transporting Polypeptide Family in Human Epidermal Keratinocytes  Ruth Schiffer, Mark.

Similar presentations

Ads by Google