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Eosinophilic gastrointestinal disease and peanut allergy are alternatively associated with IL-5+ and IL-5− TH2 responses  Calman Prussin, MD, Joohee Lee,

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Presentation on theme: "Eosinophilic gastrointestinal disease and peanut allergy are alternatively associated with IL-5+ and IL-5− TH2 responses  Calman Prussin, MD, Joohee Lee,"— Presentation transcript:

1 Eosinophilic gastrointestinal disease and peanut allergy are alternatively associated with IL-5+ and IL-5− TH2 responses  Calman Prussin, MD, Joohee Lee, MD, Barbara Foster, MS  Journal of Allergy and Clinical Immunology  Volume 124, Issue 6, Pages e6 (December 2009) DOI: /j.jaci Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Detection of food allergen–specific T-cell responses. A-D, Gating. CD4+ T-cell expression of CD154 and either IL-4 (E and G) or IL-5 (F and H) after incubation with peanut antigen (E and F) or media (G and H). In a separate experiment, peanut antigen–activated cultures were incubated with isotype control (I and J), or anti-MHC class II mAb (K and L), n = 5. SSC, Side scatter; FSC, foward scatter. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 AEG is associated with a greater frequency of peanut-specific IL-5–producing T cells. The frequency of (A) IL-4, (B) IL-5, (C) IFN-γ, and (D) TNF-expressing peanut antigen–specific CD4 cells was determined for each subject group. Each symbol represents an individual subject. The median value is denoted by a horizontal bar. Intergroup statistics are shown over the brackets. NA, Nonatopic. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Food-specific T cells exhibit complex cytokine coexpression patterns. Cytokine coexpression by peanut-specific T cells from subjects with AEG (A, C-E) or PA (B). F, Each of the 15 possible cytokine combinations is shown as a proportion of the total peanut response. Individual subjects and medians are denoted by dots and horizontal bars, respectively. All subjects from the 3 groups were studied. ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ NA, Nonatopic. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 AEG is singularly associated with food allergen–specific IL-5+ TH2 cells. A-D, The frequency of food antigen (Ag)–specific CD4 cells for each cytokine subpopulation and subject group. E-H,Stack graphs depict the median values for each cytokine subpopulation summed to represent the total frequency of Ag-specific cytokine-producing cells. NA, Nonatopic; SEB, staphylococcal enterotoxin B. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Correlation of TH2 responses with IgE and eosinophilia. A, Correlation of IL-5+ TH2 cells with eosinophil count. Correlation of peanut-specific IgE with IL-5– TH2 (B) and IL-5+ TH2 (C) cells. D, Correlation of soy-specific IgE with IL-5– TH2 cells. Linear regression curve fit is shown for subjects with AEG (A) and PA (B and C). SEB, Staphylococcal enterotoxin B. ∗P < .05. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Peanut antigen–specific CD4 T-cell cytokine responses
Peanut antigen–specific CD4 T-cell cytokine responses. PBMCs from all 4 subject groups were incubated with either media or peanut antigen for 6 hours, and the frequency of CD154+, cytokine-producing cells was determined by flow cytometry. Each symbol represents a subject with AEG. Red symbols and lines denote the 2 subjects with AEG treated with prednisone. The bracketed numbers at the base of some plots denote the number of overlapping 0 value results. NA, Nonatopic. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Ratiometric analysis of peanut antigen–specific cells
Ratiometric analysis of peanut antigen–specific cells. The frequency of antigen-specific cytokine-producing cells as shown in Fig 2 was used to calculate IL-4:IFN-γ (A) and IL-5:IFN-γ (B) ratios for each subject. Each symbol represents a unique subject. NA, Nonatopic. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 AEG is singularly associated with food allergen–specific IL-5+ TH2 cells. Pie graphs represent the proportional contribution of each cytokine subpopulation to the total antigen-specific response. Each pie corresponds to the same lettered stack graph in Fig 4, E-H. NA, Nonatopic; SEB, Staphylococcal enterotoxin B. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

10 Correlation of IL-5+ TH2 cells to tissue and blood eosinophilia
Correlation of IL-5+ TH2 cells to tissue and blood eosinophilia. A-C, The correlation between food antigen (Ag)–specific IL-5+ TH2 cells and blood eosinophil count. D-G, The correlation between staphylococcal enterotoxin B–specific IL-5+ TH2 cells and gut tissue eosinophilia. Gut tissue eosinophilia was measured as the upper quartile count of eosinophils (eos)/high power field. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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