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Example fluorescence microscopy traces of fluorescein uptake in the presence and absence of a representative OAT inhibitor, methotrexate. Example fluorescence microscopy traces of fluorescein uptake in the presence and absence of a representative OAT inhibitor, methotrexate. During an uptake trial, the signal trace shows an initial rapid increase in fluorescence due to the change-out of chamber with fluid containing FL (a), followed by a slower increase representing accumulation in the tubule (b). Douglas S. G. Neufeld et al. J Exp Biol 2005;208: © The Company of Biologists Limited 2005
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