1. Fig. 4. Targeted disruption of STK35 transcripts in mouse.
Targeted disruption of STK35 transcripts in mouse. (A) Gene targeting strategy for STK35 KO mice. After homologous recombination, exons 1, 2 and 3 were replaced by a FRT-flanked neomycin (Neo) cassette. PCR primers for genotyping are shown as Ps1, Ps2 and Ps3. (B) Southern blot analysis of XbaI-digested DNA isolated from ES clones. Recombination of the 3′-ends detected as a change of a 7.8 kb WT XbaI fragment to a novel 5.9 kb fragment using the indicated 3′ probe. (C) PCR analysis using genomic DNA of the indicated genotypes. (D) Genetic analysis of pups generated from heterozygous parents. A total 185 mice were counted, with the sex ratio of each genotype recorded. (E) Uteri isolated from STK35 Het mice at embryonic 12.5 day (E12.5; a,c) and E13.5 (b,d), in which the exhibited embryos (c,d) were obtained from uteri (a,b), respectively. Dead embryos are indicated by black arrows. Scale bars: 5 mm. (F) In situ hybridization analysis of E13.5 whole WT (+/+) embryos using probe P2/3. (G) Body weight of male and female mice (>40 dpp) for each genotype (n=8 each).
Yoichi Miyamoto et al. Biology Open 2018;7:bio032631
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