1. Pitx3-GFP+ cells exhibit functional neuron-like electrophysiological properties.
Pitx3-GFP+ cells exhibit functional neuron-like electrophysiological properties. (A) DIC and GFP image showing a recording pipette patched onto a mouse Pitx3-GFP+ cell. (B) Spontaneous action potentials fired by a day-14 neuron. Vertical scale bar, 20 mV; horizontal scale bar, 5 mseconds. (C) A single spontaneous action potential (left). A single action potential fired in response to a 50 pA depolarizing current pulse (middle). Multiple action potentials fired in response to a 50 pA depolarizing current pulse (right). Vertical bars, 20 mV; horizontal bars, 50, 100 and 100 mseconds, respectively. (D) Outward current evoked by a serious of voltage steps (left). Fast inward current evoked by a series of voltage steps (right). Vertical bars, 1 nA; horizontal bars, 50 mseconds. (E) Pacemaker-like spontaneous firing a day-41 neuron. Vertical bar, 20 mV; horizontal bar, 1 second.
Ines Jaeger et al. Development 2011;138:
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