1. Neutralization and knockdown of TGFβ2 results in fewer nephrons ex vivo.
Neutralization and knockdown of TGFβ2 results in fewer nephrons ex vivo. (A,B) Neutralization of TGFβ2 results in fewer nephrons quantified by WT1 staining [number of nephrons (arrows in A) quantified in B; 4 µg/ml IgG versus 4 μg/ml anti-TGFβ2: 20.2 versus 7.2, n=3, *P=0.017]. (C,D) Knockdown of TGFβ2 using in vivo morpholinos results in a decrease in the number of WT1+ structures, similar to neutralization [number of nephrons (arrows in C) quantified in D; control MO treated versus splice inhibiting MO treated: 20.9 versus 13.8, n=3 controls MO and n=4 splice inhibiting MO treated, *P=0.015). Each biological replicate (n) was an average of untreated or treated explants from a different litter. Data are mean±s.e.m. Scale bars: 1000 µm in bright-field images; 220 µm in immunofluorescent images.
Christopher J. Rowan et al. Development 2018;145:dev159947
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