1. Arterial Notch signaling is not required for artery patterning.
Arterial Notch signaling is not required for artery patterning. (A) Timeline of the experimental procedure. (B) Maximum intensity projections of Isolectin B4 (blue) and α-SMA (red) stained retinal whole-mounts of Bmx(PAC)-CreERT2 × Rbpjflox/flox (RbpjiΔaEC) mutants and littermate controls at P28. Artery-specific Cre activity in the mutant was visualized by incorporation of a Rosa26-YFP reporter allele (green). Note the normal vessel patterning in the absence of arterial Rbpj. a, artery; v, vein. (C) Analysis of arterial branch points (top) showed no significant (n.s.) differences by Student’s t-test, whereas arterial Dll4 protein (bottom) was significantly reduced. Error bars indicate s.e.m. (D) Anti-Dll4 immunofluorescence (red) was strongly reduced in P28 RbpjiΔaEC mutants carrying a Rosa26-YFP allele (green). ECs are labeled by Isolectin B4 (blue).
Manuel Ehling et al. Development 2013;140:
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