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Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis. Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis.

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Presentation on theme: "Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis. Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis."— Presentation transcript:

1 Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis.
Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis. A DNA vector expressing PH-Akt-GFP was transiently transfected into HEK293 cells stably expressing either (A) WT CXCR2 or (B) LLKIL mutant CXCR2. Time-lapse fluorescence microscopic images were taken every minute immediately after a point source of ligand (1 μg/ml MIP-2) was applied. The number in each picture shows the time-lapse in minutes and the arrow indicates the direction of ligand application. The arrowheads point towards the accumulation of PH-Akt-GFP. Scale bars, 20 μm. Each experiment was repeated at least three times and representative images are shown. The time-lapse video clips are available online (Movies 1 and 2 in supplementary material). Jiqing Sai et al. J Cell Sci 2004;117: © The Company of Biologists Limited 2004

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