1. Starvation triggers the selective induction of FAM134B-2.
Starvation triggers the selective induction of FAM134B-2. (A) qRT-PCR analysis of FAM134B, SEC62, RTN3, and CCPG1 in mouse livers from fed, fasted, and re-fed conditions. (B) Immunoblot analysis of FAM134B in mouse livers (fed, fasted, and re-fed), mouse brain, and recombinant FLAG-FAM134B-1 generated in HEK293T cells. (C) Gel electrophoresis of 5′ RACE products using brain cDNA from fed mice or liver cDNA from fasted mice. (D) Schematic models of mouse FAM134B-1 and FAM134B-2 genes. (E) Co-immunoprecipitation of endogenous LC3B, FLAG-FAM134B-2, and FLAG-FAM134B-2-ΔLIR. FLAG-FAM134B-2 was transiently overexpressed in HEK293T cells. 24 h after transfection, the cells were starved for 6 h in the presence of EBSS and bafilomycin A1 (1 μM). (F) qRT-PCR analysis of FAM134B-1 and FAM134B-2 in mouse livers under fed, fasted, and re-fed conditions. (G) Immunoblot analysis of FAM134B in mouse livers (fed and fasted), recombinant FLAG-FAM134B-1 and 2 generated in HEK293T cells. One-way ANOVA with a Student–Newman post hoc test was used for statistical analysis. *P < 0.05.
Shohei Kohno et al. LSA 2019;2:e
© 2019 Kohno et al.