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Published byLesley Small Modified over 5 years ago
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I. Microscopy magnification Ocular x Objective resolving power
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Electron Microscopes:
Better resolving power than light microscopes TEM aims an electron beam at a thin stained section Used to study internal cell structure
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SEM beam scans the surface of a specimen coated with a thin layer of gold
Visualized entire image Creates a 3-D image
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DENSITY BUT, EM’s can only view dead cells
PHASE CONTRAST allow unstained, living tissues to be observed CELL FRACTIONATION using a centrifuge to separate cell components based on DENSITY
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PROKARYOTIC vs. EUKARYOTIC
found in Kingdoms Protista, Fungi, Plantae, Animalia found only in Kingdom Monera no true nucleus; lacks nuclear envelope true nucleus; bounded by nuclear envelope genetic material in nucleoid region genetic material within the nucleus
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PROKARYOTIC vs. EUKARYOTIC
contains cytoplasm w/ cytosol and membrane-bound organelles no membrane-bound organelles have a plasma membrane and usually a cell wall usually smaller than eukaryotes
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PROKARYOTIC vs. EUKARYOTIC
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II. PARTS of the CELL CELL SIZE is limited by: metabolic requirements
surface area to volume ratio II. PARTS of the CELL
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nucleus histones
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cytoplasm cytosol nucleolus ribosomes endoplasmic reticulum
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Golgi apparatus lysosomes intracellular digestion apoptosis
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vacuoles peroxisomes
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mitochondria
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Cytoskeleton microtubules intermediate filaments microfilaments
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cell walls
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glycocalyx Intercellular Junctions tight junctions desmosomes
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gap junctions
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IV. MEMBRANES Singer-Nicolson Fluid Mosaic Model
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Selective Permeability
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Water Potential hypoosmotic environments isotonic environments hyperosmotic environments
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Diffusion and Osmosis
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Facilitated Diffusion
Active Transport
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Forms of Active Transport
ATP Pump Symport Antiport
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Receptor-mediated endocytosis
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