1. Nuclear DNA signal is altered in Chd5-deficient NSCs.
Nuclear DNA signal is altered in Chd5-deficient NSCs. (A) Representative immunofluorescent images of wild-type (+/+) and Chd5-deficient (Chd5−/−) NSCs that were undifferentiated (0 h) or differentiated (for 6, 12, 24 and 48 h) and assessed for DAPI nuclear signal (left panels) and corresponding grayscale images (right panels). Dashed rectangles indicate the zoomed-in regions, shown in insets. Scale bar = 50 μm (main image) and 10 μm (insets). (B) Temporal representation of mean signal intensities over pixels of DAPI nuclear signal of wild-type (blue) and Chd5-deficient (gray) NSCs, as plotted in Fig S2C and D. Data are represented as mean ± SD (n = 2). (C, D) Distribution and comparison of mean signal intensities over pixels of DAPI signal in randomly selected fields (9–16 fields per sample) of wild-type (upper panels) and Chd5-deficient (lower panels) NSCs at all time points, indicated in representative images in Fig S2A. Data are represented as mean ± SD (n = 2). ns, no significance; ****<0.0001; Tukey’s multiple comparisons test.
Dong-Woo Hwang et al. LSA 2018;1:e
© 2018 Hwang et al.