1. Treatment of dogs with celecoxib interferes with the effect of low-dose aspirin on ex vivo platelet aggregation.
Treatment of dogs with celecoxib interferes with the effect of low-dose aspirin on ex vivo platelet aggregation. Purpose-bred beagle dogs (n = 6, 10–12 kg) were administered low-dose aspirin alone (1.16 mg/kg, p.o.; LD ASA), celecoxib alone (1.43 mg/kg, po bid; CBX), or both celecoxib plus low-dose aspirin (CBX + LD ASA) for a period of three days. At the conclusion of each treatment regimen, platelet-rich plasma was prepared by centrifugation from venous whole blood collected in 3.7% sodium citrate. Ex vivo platelet aggregation responses to three platelet agonists [AA (650 μM), adenosine diphosphate (20 μM), and γ-thrombin (70 nM)] were recorded. Data are expressed as the mean ± SEM. *** indicates p < 0.001 when each time point is compared to the same agonist at day 0 by two-way ANOVA.
Gilad Rimon et al. PNAS 2010;107:1:28-33
©2010 by National Academy of Sciences