1. Chapitres Choisis english open book exam: pass / fail 4 week blocks of different topics yeast genetics.... cell biology of lipids

2. Genetics, Molecular and Cell Biology of Yeast 1.Introduction 2.Genetic elements 3.Classical genetics 4.Molecular biology of yeast 5.Cell biology 6.Genomics

3. Roger Schneiter January 2004

6. What are Yeast ? –eukaryote –unicellular fungi –phylum Ascomycetes –order Saccharomycetales –wide dispersion of natural habitats, plant leaves, wine grapes –ferment sugars to ethanol Production of wine, beer etc. Baking, raise dough through production of CO 2 Sparkling wine Used by man aprox. 6000 years B.C. –difficult to define a wild-type –vitamin supplement in food production –divide by budding; Saccharomyces (cerevisiae) –divide by division: Schizosaccharomyces (pombe) –human pathogen: Candida albicans

7. Yeast as a model eukaryote the awesome power of yeast genetics -> the awesome power of yeast cell biology Fast generation cycle (90 min) Non-pathogenic Chemically defined growth media Large quantities -> class. biochemistry Amenable to classical genetics Large mutant collections Strong cell biology Strong -omics (gen-, proteo-, metabol-,...

8. Transformation with rec. DNA Extremely efficient recombination Molecular biology methods unique to yeast Complete sequence of genome DNA Arrays Complete knock-out collections Complete protein interaction maps

9. The power of genetics Leland Hartwell, Nobel Prize 2001 the cdc-screen

10. Ask an important question !

11. Life cycle

13. pseudohyphae

16. Pheromone binds Kinase cascade Ste12p phosphorylated Activated Ste12p starts mating program Signal cascade

17. Cell polarity of diploid vs haploid cells