1. Sparse excision of PirB at E15
Sparse excision of PirB at E15.5 increases the mEPSC frequency of targeted L2/3 pyramidal neurons in visual cortex, but not in unmanipulated neighboring cells.
Sparse excision of PirB at E15.5 increases the mEPSC frequency of targeted L2/3 pyramidal neurons in visual cortex, but not in unmanipulated neighboring cells. A, B, Combined differential interference contrast and fluorescence micrograph of visual cortical slice used for whole-cell recordings, showing electrode targeting of a GFP−;Cre− cell (A) or an isolated GFP+;Cre+ cell (B). C, D, Example traces of mEPSC events in GFP−;Cre− (C) or GFP+;Cre+ (D) L2/3 pyramidal neurons in slices of visual cortex from P30 PirBfl/fl mice. E, In PirBfl/fl mice, GFP+;Cre+ cells have higher mEPSC frequency compared with GFP−;Cre− cells (GFP−;Cre−: ± 2.38 Hz, n = 16 cells, 10 slices, 7 mice; GFP+;Cre+: ± 4.60, n = 16 cells, 10 slices, 7 mice; p = l, Wilcoxon signed-rank test). F, mEPSC amplitude is not significantly different in GFP−;Cre− vs GFP+; Cre+ cells (GFP−;Cre−: ± 1.01 pA, n = 16 cells, 10 slices, 7 mice; GFP+;Cre+: ± 0.77, n = 16 cells, 10 slices, 7 mice; p = m, Wilcoxon signed-rank test). G, H, Cumulative histograms (by cell) of data presented in E and F, respectively. Scale bars: A, B, 25 μm. **p < 0.01
George S. Vidal et al. eneuro 2016;3:ENEURO
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