1. Identification of the uvh6-4 mutation.
Identification of the uvh6-4 mutation. (A) Heat stress–induced activation of the L5-GUS transgene in rosette leaves of WT (L5 background) and mutants after 24 h at 37°C was detected by β-glucuronidase (GUS) staining. (B) RT-qPCR analysis of transcripts from L5-GUS and MULE, normalized to the reference gene AT5G12240 and further normalized to the mean of WT samples at 23°C. Errors bars represent standard error of the mean across two biological replicates. Statistically significant differences between means of uvh6-3 and uvh6-4 samples at 37°C were tested using unpaired unilateral t tests (*P < 0.05, **P < 0.005). (C) Representative pictures of 16-d-old seedlings of the indicated genotypes grown in soil and in long day conditions. Scale bar: 1 cm.
Pierre Bourguet et al. LSA 2018;1:e
© 2018 Bourguet et al.