1. Monitoring the acetylation profile of mitochondrial proteins in SIRT3 KO mice.
Monitoring the acetylation profile of mitochondrial proteins in SIRT3 KO mice.A, schematic of workflow for acetylpeptide enrichment from skeletal muscle mitochondria from WT and SIRT3 KO animals, including anti-acetyllysine enrichment of Lys-acetylated peptides, LC-MS/MS of two injection replicates, and generation of a spectral library in Skyline for MS1 filtering and relative quantitation. B, ratio (KO:WT) of peak area intensities for individual tryptic acetyllysine peptides at different sites across three proteins from skeletal muscle samples for four WT and four SIRT3 KO mice. # indicates significance: p < 0.05 by two-tailed Student's t test; n = 4 for WT and SIRT3−/− (KO) with two injection replicates per sample.
Birgit Schilling et al. Mol Cell Proteomics 2012;11:
© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.