1. Characterization of messenger RNA expression of estrogen receptor-α and -β in patients with ovarian endometriosis 
Sachiko Matsuzaki, M.D., Takao Fukaya, M.D., Shigeki Uehara, M.D., Takashi Murakami, M.D., Hironobu Sasano, M.D., Akira Yajima 
Fertility and Sterility 
Volume 73, Issue 6, Pages (June 2000)
DOI: /S (00)

2. Figure 1
Schematic representation of the structures of the ER-α– and ER-β–coding regions (complementary DNA) and the locations of the primers used for RT-PCR analysis. The positions of the translation initiation (ATG) and termination (TGA) codons are indicated. Black rectangles indicate the primer locations. Exon boundaries are shown as vertical bars. F = forward primer; R = reverse primer.
Matsuzaki. ER-α and -β in endometriosis. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )

3. Figure 2
The RT-PCR detection of ER-α and ER-β mRNA in two samples of ovarian endometriotic tissues. The molecular sizes of the specific bands are indicated. Bands, of which molecular sizes were <100 bp, were derived probably from primer dimers. (A), from case no. 6 and (B) from case no. 19 in Table 3. M = molecular size marker (100-bp ladder); lane 1, RT-PCR for ER-α mRNA; lane 2, for ER-β mRNA; lane 3, for β-actin mRNA; and lane 4, negative control without RT.
Matsuzaki. ER-α and -β in endometriosis. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )

4. Figure 3
In situ localization of ER-α and ER-β mRNA in an ovarian endometriotic cyst obtained from a patient (case no. 6 in Table 3). The ISH was performed on adjacent sections of an ovarian endometriotic cyst with an antisense probe to ER-α (a) and an antisense probe to ER-β (b). Both ER-α and ER-β mRNA hybridization signals, appearing red as a result of fast red, were detected in glandular epithelial and stromal cells. Negative controls with a sense probe to ER-α (c) or a sense probe to ER-β (d) showed no detectable specific mRNA hybridization signals. Bar = 50 μm.
Matsuzaki. ER-α and -β in endometriosis. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )

5. Figure 4
In situ localization of ER-α and ER-β mRNA in an ovarian endometriotic cyst obtained from a patient (case no. 19 in Table 3). The ISH was performed on adjacent sections of an ovarian endometriotic cyst with an antisense probe to ER-α (a) and an antisense probe to ER-β (b). The ER-α hybridization signals, appearing red as a result of fast red, were detected in glandular epithelial and stromal cells. No ER-β hybridization signals were detected. Bar = 50 μm.
Matsuzaki. ER-α and -β in endometriosis. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )