1. Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for BJ Cells (Skin Fibroblast Cells, CRL-2522)
Altogen Biosystems offers the BJ Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
The BJ Transfection Reagent is a nanoparticle-based liposome formulation reagent that enhances lipid-mediated transfection, and it has been developed to provide high transfection efficiency with BJ cells.
This cell line is an excellent host for transfection and the assessment of cellular functionality. When cultured in vitro, BJ cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase BJ Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. BJ Cell Line Description and Applications
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for BJ Cells (Skin Fibroblast Cells, CRL-2522)
BJ Cell Line Description and Applications
The BJ cell line originates from the foreskin of a healthy newborn male. The BJ cell can be characterized as a fibroblastic cell line; however, this cell line has a longer lifespan in comparison to other fibroblast cells. The BJ cell line has applications as an efficient transfection host and as well as a tool for assessing cellular functionality. The BJ cell line can achieve up to 72 population doublings before senescence. Research indicates the cell line has a standard diploid karyotype at population doubling 61 and an abnormal karyotype at 82. The cell line is telomerase negative.
BJ Cells
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. BJ Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for BJ Cells (Skin Fibroblast Cells, CRL-2522)
BJ Transfection Protocol
1. Plate 10, ,000 BJ cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however, it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with BJ cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of BJ cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for BJ Cells (Skin Fibroblast Cells, CRL-2522)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

5. BJ Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for BJ Cells (Skin Fibroblast Cells, CRL-2522)
BJ Transfection Kit Product Details
A nanoparticle-based liposome formulation
Transfection protocols provided for transfection of proteins, DNA, mRNA, siRNA, shRNA and microRNA
Transfection Enhancer reagent provided with the kit
Produce higher level of recombinant protein expression with minimal disruption of normal cell function
Generate physiologically relevant data you can trust4
Effective for plasmid DNA/siRNA co-transfection
Easy-to-use transfection protocol with reproducible results
Low cytotoxicity
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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6. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for BJ Cells (Skin Fibroblast Cells, CRL-2522)
Data
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in BJ cell line transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).

7. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for BJ Cells (Skin Fibroblast Cells, CRL-2522)
Data
Figure 2. Protein expression of Cyclophilin B in BJ cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into BJ cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. BJ Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for BJ Cells (Skin Fibroblast Cells, CRL-2522)
BJ Transfection Kit Benefits
Pre-optimized transfection protocol for BJ cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use BJ transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable BJ cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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