1. Production of lentiviral vectors
Otto-Wilhelm Merten, Matthias Hebben, Chiara Bovolenta 
Molecular Therapy - Methods & Clinical Development 
Volume 3, (January 2016)
DOI: /mtm
Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy Terms and Conditions

2. Figure 1
One example of the third generation Tat-independent HIV-1 vector system. The shown SIN transfer vector contains the cPPT for efficient nuclear import and uses the MSCV LTR promoter (MU3) as internal promoter for driving the expression of the transgene, as well as the WPRE (W) element for high-level transgene expression. The three other packaging constructs encode for the HIV-1 gag-pol and rev proteins as well as for the VSV-g envelope glycoprotein.20 SIN, self-inactivating; VSV-g, glycoprotein of the vesicular stomatitis virus.
Molecular Therapy - Methods & Clinical Development 2016 3, DOI: ( /mtm )
Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy Terms and Conditions

3. Figure 2 Ten stack Cell Factory from Nunc (CF-10).
Molecular Therapy - Methods & Clinical Development 2016 3, DOI: ( /mtm )
Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy Terms and Conditions

4. Figure 3
Principle process steps of large-scale downstream processing protocols for the purification of VSV-g-pseudotyped LV vectors (for clinical purposes). The company/institution name indicated in red informs that their downstream processing protocol makes use of ion-exchange (IEX) chromatography. (?)'no details are available on the process step (e.g., with respect to the filtration step, the pore size/exclusion size was not communicated). Sterilization'sterile filtration (0.2 µm). *A similar purification process however, devoid of the benzonase and the following diafiltration step was used by Greene et al.56 for the purification of clinical material produced with stable producer cell lines. **The protocol published by Ausubel et al. 43 does not use a final sterile filtration step, thus each batch/sub-batch requires separate testing for sterility before final processing and further use. LV, lentiviral vectors; SEC, size exclusion chromatography; TFF, tangential flow filtration; VSV-g (glycoprotein of the vesicular stomatitis virus).
Molecular Therapy - Methods & Clinical Development 2016 3, DOI: ( /mtm )
Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy Terms and Conditions