1. BT_0370 galactokinase and BT_371 glucose/galactose transporter
BT_0370 galactokinase and BT_371 glucose/galactose transporter Selection kinetics by fragments per kilobase mapped (FPKM) fold change and normalized effective coverage of genes BT_0368, BT_0369, BT_0370, BT_0371, and BT_0372.Mapped reads to each base in the region with deep sequencing and Sanger sequencing of isolated clones (below). Read values are the mean across five mice and were normalized to 1 billion mapped bases per run to compare across time points. Isolation of individual clones allowed for insert size profiling at Day 7. Screened isolates from Day 28 did not reveal any galactokinase inserts.Functional characterization in minimal media with galactose as the sole carbon source. Three sets of strains were studied: (i) starting E. coli strains transformed with the CDS of each gene cloned into the backbone vector, (ii) E. coli clones directly isolated from stool samples, and (iii) starting E. coli strains re‐transformed with individual plasmids isolated from stool samples. All clones isolated at Day 28 carried the BT_1759 locus. Lines represent the mean.Genotyping of the background E. coli genome at the galK locus. 20 clones isolated from mice inoculated with the library at each of the indicated time points were screened, while 30 clones isolated from the lux control mice at Day 28 were screened. Source data are available online for this figure.
Stephanie J Yaung et al. Mol Syst Biol 2015;11:788
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