1. HPLC-ESI-QqTOF MS
Lacková Zuzana
Brno

2. Fig. 2: maXis impact weight and dimensions
Fig. 1: LC/MS data system arrangement

3. Fig. 4: Principle of the ESI process
Fig. 3: Source (spray chamber and capillary), Ion Transfer Stage (funnel 1, funnel 2 , hexapole), Q-q-Stage (quadrupole, collision cell) and TOF spectrometer (orthogonal accelerator, reflector, detector)
Fig. 4: Principle of the ESI process

4. Fig. 6: Double Stage Ion Funnel and Hexapole
Fig. 4: API interface
Fig. 5: Desolvation unit
Fig. 6: Double Stage Ion Funnel and Hexapole
Fig. 7: Analytical Quadrupole and Collision Cell (Q-q-Stage)
Fig. 8: TOF mass analyzer

5. CURRENT STATE AND AIMS FOR THE FUTURE
absorption maximum at nm
Blanc (water)
problem with impurities suppressing analytes
problem with sample spraying
problem with dirt in the source
Gallic acid (50 µg/ml)
Gallic acid (10 µg/ml)
Fig. 9: Chromatogram from HPLC-PDA analysis

6. CURRENT STATE AND AIMS FOR THE FUTURE
absorption maximum at nm
Blanc (water)
column: Zorbax Eclipse AAA (4.6x150 mm; 3.5 µm)
mobile phase A: water + 0.1% HCOOH, mobile phase B: methanol + 0.1% HCOOH
gradient: 0 min 10% B → 15 min 70% B → 20 min 100% B → 22 min 100% B → min 10% B → 30 min 10% B (STOP).
Gallic acid (50 µg/ml)
Gallic acid (10 µg/ml)
Fig. 10: Chromatogram from HPLC-PDA analysis
analysis of GSH, GSSG, SAM and SAH in urine samples

7. acknowledgment
Mgr. Roman Guráň, Ph.D.
RNDr. Ondřej Zítra, Ph.D.

8. Thank you for your attention14