1. Differential Expression of E Prostanoid Receptors in Murine and Human Non-Melanoma Skin Cancer 
Juliette Lois Lee, Arianna Kim, Levy Kopelovich, David R. Bickers, Mohammad Athar 
Journal of Investigative Dermatology 
Volume 125, Issue 4, Pages (October 2005)
DOI: /j X x
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Differential expression of E prostanoid receptors in normal and chronic ultraviolet B (UVB)-irradiated murine skin. Immunohistochemical analysis was performed in skin obtained from SKH-1 mice. Chronic UVB-irradiated skin was obtained from mice exposed to 180 mJ per cm2 UVB twice weekly for 30 wk or greater, whereas control skin was obtained from non-irradiated, age-matched controls.
Journal of Investigative Dermatology  , DOI: ( /j X x)
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
E prostanoid receptors are expressed in murine ultraviolet B (UVB)-induced benign papillomas and squamous cell carcinomas (SCC) but not in UVB-induced basal cell carcinomas (BCC). Immunohistochemical analysis was performed on benign papillomas and SCC from SKH-1 mice and BCC from Ptch1+/- mice subjected to a standard photocarcinogenesis protocol. Areas designated as T represent BCC tumor islands, whereas red arrows indicate positive staining in overlying epidermis. Scale bars (A, B)=100 μm and (C)=50 μm.
Journal of Investigative Dermatology  , DOI: ( /j X x)
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Differential expression of E prostanoid receptor mRNA in ultraviolet B (UVB)-induced benign papillomas and squamous cell carcinomas (SCC) in SKH-1 mice. (A) RT-PCR analysis was performed on five samples each of control skin, benign papillomas, and SCC harvested from SKH-1 mice subjected to a standard photocarcinogenesis protocol. Unirradiated mice served as controls. (B) Densitometric analysis demonstrated that these changes were statistically significant. *p-value <0.05 when compared with normal skin using Student's t test.
Journal of Investigative Dermatology  , DOI: ( /j X x)
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
E prostanoid receptors are expressed in human actinic keratosis (AK), Bowen's disease, and squamous cell carcinomas (SCC), but not in basal cell carcinomas (BCC). Immunohistochemical analysis was performed on histologic sections of sporadic human AK, Bowen's disease, SCC, and BCC from sun-exposed sites. Areas designated as T represent BCC tumor islands, whereas red arrows indicate positive staining in overlying epidermis. Scale bars (A, D)=100 μm and (B, C)=50 μm.
Journal of Investigative Dermatology  , DOI: ( /j X x)
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
E prostanoid (EP) receptor mRNA levels are significantly increased in human squamous cell carcinomas (SCC) but not basal cell carcinomas (BCC) as compared with adjacent non-tumor skin. Quantitative assessment of EP receptor mRNA levels using real-time PCR in five human SCC and BCC tumor samples as compared with adjacent non-tumor-bearing skin from the same patient, with non-tumor-bearing skin normalized to value 1. *p-value <0.05 when compared with adjacent tumor-free skin using Student's paired t test.
Journal of Investigative Dermatology  , DOI: ( /j X x)
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions