1. Do Now 9/18
On a separate piece of paper, make a list of words you think should not be used on the AICE exam
WOD: ambiguous (adjective)
(of language) open to more than one interpretation; having a double meaning.

2. “You” Or really any other pronouns for that matter
“You can’t see a ribosome with a light microscope.”

3. “You” Or really any other pronouns for that matter
“You can’t see a ribosome with a light microscope.”
“Ribosomes cannot be seen using light microscopy.”

4. “Things” and “Stuff” Too ambiguous. What “things”? What “stuff”?
“The cell surface membrane is responsible for keeping stuff inside or outside of the cell”
“Nuclear pores let things in and out of the nucleus.”

5. “Things” and “Stuff” Too ambiguous. What “things”? What “stuff”?
“The cell surface membrane is responsible for keeping stuff inside or outside of the cell”
“The CSM is responsible for regulating what molecules enter and exit the cell.”
“Nuclear pores let things in and out of the nucleus.”
“Nuclear pores allow for particles, like ribosomes and mRNA to exit the nucleus, and for particles like hormones and nucleotides to enter the nucleus.

6. “Things” and “Stuff”
Words to use instead: molecules, macromolecules, the actual name for the “thing”, structure, vesicle

7. “Good” and “Bad”
Less ambiguous, but not appropriate for Cambridge level students in a formal paper
Good: beneficial, necessary
Bad: harmful, unwanted
What is considered “good” to one cell might be “bad” for another cell, so avoid these two words at all costs!

8. “Job”
Cells and organelles don’t have jobs (They can’t quite figure out how to type a resume yet)
“The job of the ribosome is to synthesize proteins”

9. “Job”
Cells and organelles don’t have jobs (They can’t quite figure out how to type a resume yet)
“The job of the ribosome is to synthesize proteins”
“The function of the ribosome is to synthesize proteins.”

10. Shape
Like “good” and “bad” it’s not that this word is ambiguous, but it is generally not used in Biology
Instead: structure, complex, organization

11. Metaphor, Simile, and Analogy
Fine for literature, but not for AICE Biology exams
“The mitochondria is the powerhouse of the cell.”
“The nucleus is like the brain of the cell.”

12. Metaphor, Simile, and Analogy
Fine for literature, but not for AICE Biology exams
“The mitochondria is the powerhouse of the cell.”
The mitochondria creates chemical energy for the cell in the form of ATP.
“The nucleus is like the brain of the cell.”
The nucleus controls the functions of the cell by transcribing DNA

13. Other words to avoid
A lot
Many
Really
Very
Lots
Nice

14. Prelab discussion Practical 1.1 Practical 1.2
A: Calibrating an eyepiece graticule and B: Preparing a slide of onion epidermal cells
Practical 1.2
Preparing a slide of human cheek cells

15. Safety Precautions Goggles and gloves must be worn at all times
Glass slides will be used, follow all glassware safety precautions
Cotton buds, slides and cover slips must be placed in disinfectant after use.
Care should be taken in using mains-operated microscopes with water/solutions.
Normal lab safety precautions should be taken with sharps and chemicals

16. Practical 1.1 In this practical, you will:
• make a temporary preparation of some onion epidermal cells
• stain the cells so that you can see structures within them
• set up a light microscope and use it to make observations and measurements
• make a drawing of the onion cells
• calibrate an eyepiece graticule and use it to measure the size of the cells.

17. Practical 1.2 In this practical, you will:
• make a temporary preparation of human cheek cells
• stain the cells so that you can see structures within them
• set up a light microscope and use it to make observations and measurements
• make a drawing of the cheek cells
• use an eyepiece graticule to measure the size of the cells.

18. Prelab questions What is the limit of resolution for light microscopy?
What units are measured using an eyepiece graticule?
Why do we use methylene blue (a deep blue/violet stain) to stain epithelial cells?
What are 3 structures will see in both practicals? What are 2 structures we will see in practical 1.1 only?