Presentation is loading. Please wait.

Presentation is loading. Please wait.

Expression of Perilipin A on the Surface of Lipid Droplets Increases along with the Differentiation of Hamster Sebocytes In Vivo and In Vitro  Noriko.

Published byEileen Nicholson Modified over 5 years ago

Similar presentations

Presentation on theme: "Expression of Perilipin A on the Surface of Lipid Droplets Increases along with the Differentiation of Hamster Sebocytes In Vivo and In Vitro  Noriko."— Presentation transcript:

1 Expression of Perilipin A on the Surface of Lipid Droplets Increases along with the Differentiation of Hamster Sebocytes In Vivo and In Vitro  Noriko Akimoto, Takashi Sato, Chikakazu Iwata, Masayuki Koshizuka, Fusatoshi Shibata, Ayako Nagai, Michihiro Sumida, Akira Ito  Journal of Investigative Dermatology  Volume 124, Issue 6, Pages (June 2005) DOI: /j X x Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Expression of perilipin in sebaceous glands. Oil red O staining (A) shows lipid deposition in sebaceous glands and ducts of auricles in hamster skin. Immunohistochemical staining shows that perilipin is expressed mainly in sebaceous glands (B and D), in contrast to negative control using non-immune rabbit IgG (C). Scale bars=100 (A–C) and 50 μm (D). Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Localization of perilipin on the surface of lipid droplets in cultured hamster sebocytes. Oil red O staining (A) of hamster sebocytes at the second passage cultured for 12 d. Immunostaining of perilipin (B) shows that the protein is visualized as rings around the periphery of intracellular lipid droplets of various sizes in hamster sebocytes. Scale bars=20 μm. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Regulation of perilipin A production and mRNA expression in hamster sebocytes. Hamster sebocytes at the second passage were treated without (lane 1) or with insulin (lane 2, 10 nM), epidermal growth factor (EGF) (lane 3, 10 ng per ml), and 5α-dihydrotestosterone (5α-DHT) (lane 4, 10 nM) for 12 d, and then cell lysates and isolated RNA were subjected to western blot analysis of perilipin A (A), and to RT-PCR of perilipin A (B) (32 cycles), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA (C) (30 cycles), respectively. Four independent experiments were reproducible and typical data are shown. 3T3, differentiated mouse 3T3-L1 adipocytes. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Expression of peroxisome proliferation-activating receptors (PPARs) in hamster sebocytes. Cell lysates from untreated (lane 1)- and insulin (lane 2, 10 nM)-treated sebocytes were subjected to western blot analysis for PPARα (A) and γ (B). Note that relatively weak bands smaller than PPARα (A) and γ1 (B) are non-specific ones detected using control rabbit IgG. Four independent experiments were reproducible and typical data are shown. 3T3, differentiated mouse 3T3-L1 adipocytes. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Transcriptional augmentation of perilipin A production by ligand-activators of peroxisome proliferation-activating receptor (PPAR)α and γ in hamster sebocytes. Hamster sebocytes at the 3rd passage were treated without (lane 1) or with insulin (lane 2, 10 nM), troglitazone (lane 3, 10 μM), and WY14643 (lane 4, 100 μM) for 12 d, and then cell lysates and isolated RNA were subjected to western blot analysis of perilipin A (A) and to northern blot analysis of perilipin A (B) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA (C), respectively. Three independent experiments were reproducible and typical data are shown. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 Differential regulation of mRNA expression of perilipin A by of peroxisome proliferation-activating receptor (PPAR) activators, insulin, and 5α-dihydrotestosterone (5α-DHT) in hamster sebocytes. Hamster sebocytes at the 3rd passage were treated without (lane 1) or with troglitazone (lane 2, 10 μM), WY14643 (lane 3, 100 μM), insulin (lane 4, 10 nM), and 5α-DHT (lane 5, 10 nM) for indicated periods, and then isolated RNAs were subjected to RT-PCR of perilipin A as described in Figure 3. The relative amounts of perilipin A mRNA were quantified by densitometric scanning and expressed by taking the value for untreated cells as 100%. Data are shown as the mean±SD of three independent experiments. **Significantly different from untreated cells (p<0.01). Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Expression of Perilipin A on the Surface of Lipid Droplets Increases along with the Differentiation of Hamster Sebocytes In Vivo and In Vitro  Noriko."

Similar presentations

Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal.

Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal.
Hyaluronic Acid Decreases Lipid Synthesis in Sebaceous Glands

Hyaluronic Acid Decreases Lipid Synthesis in Sebaceous Glands
Topical Application of 17β-Estradiol Increases Extracellular Matrix Protein Synthesis by Stimulating TGF-β Signaling in Aged Human Skin In Vivo  Eui Dong.

Topical Application of 17β-Estradiol Increases Extracellular Matrix Protein Synthesis by Stimulating TGF-β Signaling in Aged Human Skin In Vivo  Eui Dong.
A Citrus Polymethoxy Flavonoid, Nobiletin Inhibits Sebum Production and Sebocyte Proliferation, and Augments Sebum Excretion in Hamsters  Takashi Sato,

A Citrus Polymethoxy Flavonoid, Nobiletin Inhibits Sebum Production and Sebocyte Proliferation, and Augments Sebum Excretion in Hamsters  Takashi Sato,
Expression of Protease-Activated Receptor-2 in SZ95 Sebocytes and its Role in Sebaceous Lipogenesis, Inflammation, and Innate Immunity  Sang E. Lee, Ji-Min.

Expression of Protease-Activated Receptor-2 in SZ95 Sebocytes and its Role in Sebaceous Lipogenesis, Inflammation, and Innate Immunity  Sang E. Lee, Ji-Min.
Human Skin is a Steroidogenic Tissue: Steroidogenic Enzymes and Cofactors Are Expressed in Epidermis, Normal Sebocytes, and an Immortalized Sebocyte Cell.

Human Skin is a Steroidogenic Tissue: Steroidogenic Enzymes and Cofactors Are Expressed in Epidermis, Normal Sebocytes, and an Immortalized Sebocyte Cell.
Decreased Expression of Caveolin-1 Contributes to the Pathogenesis of Psoriasiform Dermatitis in Mice  Yukie Yamaguchi, Yuko Watanabe, Tomoya Watanabe,

Decreased Expression of Caveolin-1 Contributes to the Pathogenesis of Psoriasiform Dermatitis in Mice  Yukie Yamaguchi, Yuko Watanabe, Tomoya Watanabe,
Epidermal Growth Factor Induces Fibronectin Expression in Human Dermal Fibroblasts via Protein Kinase C δ Signaling Pathway  Yoshihiro Mimura, Hironobu.

Epidermal Growth Factor Induces Fibronectin Expression in Human Dermal Fibroblasts via Protein Kinase C δ Signaling Pathway  Yoshihiro Mimura, Hironobu.
Efficient TRAIL-R1/DR4-Mediated Apoptosis in Melanoma Cells by Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)  Bahtier M. Kurbanov, Christoph.

Efficient TRAIL-R1/DR4-Mediated Apoptosis in Melanoma Cells by Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)  Bahtier M. Kurbanov, Christoph.
Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes  Marcel B.M. Teunissen, Jan.

Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes  Marcel B.M. Teunissen, Jan.
Regulation of HMG-CoA Synthase and HMG-CoA Reductase by Insulin and Epidermal Growth Factor in HaCaT Keratinocytes  Ian R. Harris, Hendrik Höppner, Wilfried.

Regulation of HMG-CoA Synthase and HMG-CoA Reductase by Insulin and Epidermal Growth Factor in HaCaT Keratinocytes  Ian R. Harris, Hendrik Höppner, Wilfried.
Fetal Human Keratinocytes Produce Large Amounts of Antimicrobial Peptides: Involvement of Histone-Methylation Processes  Maria Gschwandtner, Shaomin Zhong,

Fetal Human Keratinocytes Produce Large Amounts of Antimicrobial Peptides: Involvement of Histone-Methylation Processes  Maria Gschwandtner, Shaomin Zhong,
Histamine Contributes to Tissue Remodeling via Periostin Expression

Histamine Contributes to Tissue Remodeling via Periostin Expression
EGF Upregulates, Whereas TGF-β Downregulates, the Hyaluronan Synthases Has2 and Has3 in Organotypic Keratinocyte Cultures: Correlations with Epidermal.

EGF Upregulates, Whereas TGF-β Downregulates, the Hyaluronan Synthases Has2 and Has3 in Organotypic Keratinocyte Cultures: Correlations with Epidermal.
17β-estradiol, Progesterone, and Dihydrotestosterone Suppress the Growth of Human Melanoma by Inhibiting Interleukin-8 Production  Naoko Kanda, Shinichi.

17β-estradiol, Progesterone, and Dihydrotestosterone Suppress the Growth of Human Melanoma by Inhibiting Interleukin-8 Production  Naoko Kanda, Shinichi.
Androgen Receptor Co-Activator Hic-5/ARA55 as a Molecular Regulator of Androgen Sensitivity in Dermal Papilla Cells of Human Hair Follicles  Shigeki Inui,

Androgen Receptor Co-Activator Hic-5/ARA55 as a Molecular Regulator of Androgen Sensitivity in Dermal Papilla Cells of Human Hair Follicles  Shigeki Inui,
Osteopontin Expression Correlates with Melanoma Invasion

Osteopontin Expression Correlates with Melanoma Invasion
IGF-II-Mediated COX-2 Gene Expression in Human Keratinocytes Through Extracellular Signal-Regulated Kinase Pathway  Hye Jung Kim, Tae-Yoon Kim  Journal.

IGF-II-Mediated COX-2 Gene Expression in Human Keratinocytes Through Extracellular Signal-Regulated Kinase Pathway  Hye Jung Kim, Tae-Yoon Kim  Journal.
Raija Tammi  Journal of Investigative Dermatology 

Raija Tammi  Journal of Investigative Dermatology 
Enhancing 1α-Hydroxylase Activity with the 25-Hydroxyvitamin D-1α-Hydroxylase Gene in Cultured Human Keratinocytes and Mouse Skin  Tai C. Chen, Xue Hong.

Enhancing 1α-Hydroxylase Activity with the 25-Hydroxyvitamin D-1α-Hydroxylase Gene in Cultured Human Keratinocytes and Mouse Skin  Tai C. Chen, Xue Hong.

Similar presentations

Ads by Google