1. Volume 127, Issue 2, Pages 582-594 (August 2004)
Spasmolytic polypeptide expressing metaplasia to preneoplasia in H. felis-infected mice 
Sachiyo Nomura, Tammy Baxter, Hirokazu Yamaguchi, Charles Leys, Andrey B. Vartapetian, James G. Fox, Jeffrey R. Lee, Timothy C. Wang, James R. Goldenring 
Gastroenterology 
Volume 127, Issue 2, Pages (August 2004)
DOI: /j.gastro

2. Figure 1
In situ hybridization for TFF2 and SPEM-related transcripts. In situ hybridization of 11 up-regulated genes in SPEM and TFF2 was evaluated in both normal and 10-month H. felis-infected mouse fundic mucosa. TFF2 was expressed in the proliferating zone in the normal fundus and is strongly expressed in SPEM in the H. felis-infected stomach. All the analyzed genes were expressed in SPEM. Only the unknown EST gene transcript was also expressed in surface cells of H. felis-infected fundus. In the normal fundus, STY, proteasome subunit, KIAA0663, GAS5, YT521-B, NOP56, and peroxiredoxin 4 were expressed in both the chief cells and the surface mucous cells. Xist was not expressed in the normal fundus from a male mouse. Prothymosin α was expressed in almost all of the epithelial cells in the normal fundus. Rps4X was expressed most abundantly in the proliferating zone and was also expressed in the chief cells. The unknown EST transcript was expressed mainly in the surface mucous cells and was slightly expressed in the chief cells. With the exception of prothymosin α, none of the genes appeared to have significant expression in normal parietal cells. None of the corresponding sense probes demonstrated significant staining. Bar = 50 μm.
Gastroenterology  , DOI: ( /j.gastro )

3. Figure 2
Expression of SPEM-related transcripts and TFF2 in regions of gastritis cystica profunda. (A) In situ hybridization for 11 transcripts up-regulated in SPEM and TFF2 was examined in regions of gastritis cystica profunda expanding into submucosal layer through lamina propria in 10-month H. felis-infected mouse stomach. TFF2 as well as 10 out of the 11 SPEM-related genes, with the exception of KIAA0663, were strongly expressed in the epithelial cell lining regions of gastritis cystica profunda. Bar = 50 μm. (B) Immunohistochemistry for TFF2, Ki-67, and cytokeratin-7 in regions of gastritis cystica profunda. TFF2 staining was noted throughout the epithelial cell lining regions of gastritis cystica profunda. Cystic regions also demonstrated prominent staining of nuclei with Ki-67, indicative of a prominent proliferative rate. Additionally, cystic epithelia stained with antibodies against cytokeratin-7, a marker for epithelial cell dedifferentiation. Bar = 25 μm.
Gastroenterology  , DOI: ( /j.gastro )

4. Figure 3
Semiquantitative PCR analysis of expression of Xist and TFF2. Expression of TFF2 and Xist transcripts compared with that for GAPDH was assayed by semiquantitative PCR from RNA collected by laser capture microdissection from deep glandular cells and surface mucous cells in uninfected control (C) and H. felis-infected (Hf) mice. The deep glandular cells from uninfected mice were chief cells, and the deep cells from H. felis-infected mice were SPEM cells. Xist transcript was detected only in the H. felis-infected deep glandular cells (SPEM). TFF2 expression was also enriched in SPEM cells. GAPDH levels were similar in all the samples. The results are representative of 3 separate experiments.
Gastroenterology  , DOI: ( /j.gastro )

5. Figure 4
Immunocytochemical staining for TFF2 and prothymosin α. Sections of fundic mucosa from uninfected (A, C, and D) and H. felis-infected mice (B, E-H) were immunostained for TFF2 (A and B) and prothymosin α (C-H). TFF2 was expressed in the mucous neck cells in the normal fundus (A) and in SPEM in the lower two thirds of the glands in the H. felis-infected fundus (B). Prothymosin α was expressed in the nuclei of cells within the neck region in the normal stomach (C and D). In H. felis-infected fundus, nuclear staining for prothymosin α was observed in SPEM cells (E and F). Epithelial cells in regions of gastritis cystica profunda were labeled strongly with antibodies against prothymosin α (G and H). Bar = 50 μm in A-C,E,G and 12.5 μm in D,F,H.
Gastroenterology  , DOI: ( /j.gastro )