1. Volume 65, Issue 6, Pages 2343-2359 (June 2004)
ACE inhibitor and angiotensin type I receptor antagonist in combination reduce renal damage in obese Zucker rats 
Jorge Eduardo Toblli, Graciela DeRosa, Gabriel Cao, Pablo Piorno, Patricia Pagano 
Kidney International 
Volume 65, Issue 6, Pages (June 2004)
DOI: /j x
Copyright © 2004 International Society of Nephrology Terms and Conditions

2. Figure 1
Monthly evolution of systolic blood pressure in each group (A).* OZR (G1) vs. all groups P < 0.01 at sixth months. Evolution of proteinuria in each group throughout the study (B). * OZR (G1) versus all groups P < ** OZR+B + I vs. all groups P < 0.01 at sixth months. OZR, obese Zucker rats.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

3. Figure 2
Important degree of glomerulosclerosis, tubular atrophy, dilatation, and protein cast in tubular lumen, along with interstitial fibrosis in untreated OZR (A). Photomicrograph illustrate renal sections from OZR treated with benazepril (B), irbesartan (C), and both drugs in combination (D), respectively. Note appreciable benefit with each treatment, but especially with the combine therapy (D). (Masson's trichrome, magnification ×100). OZR, obese Zucker rats.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

4. Figure 2
Important degree of glomerulosclerosis, tubular atrophy, dilatation, and protein cast in tubular lumen, along with interstitial fibrosis in untreated OZR (A). Photomicrograph illustrate renal sections from OZR treated with benazepril (B), irbesartan (C), and both drugs in combination (D), respectively. Note appreciable benefit with each treatment, but especially with the combine therapy (D). (Masson's trichrome, magnification ×100). OZR, obese Zucker rats.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

5. Figure 3
Representative photomicrograph from renal cortex showing α-smooth muscle actin (α-SMA) immunostaining in all groups. Intense peritubular staining in untreated OZR (A). Appreciable reduction in the interstitial amount of α-SMA in animals treated with benazepril (B), irbesartan (C), and mostly with combined therapy (D). Note in this last group that the vascular wall only presents positive immunostaining (anti–α-SMA, magnification ×400). OZR, obese Zucker rats.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

6. Figure 3
Representative photomicrograph from renal cortex showing α-smooth muscle actin (α-SMA) immunostaining in all groups. Intense peritubular staining in untreated OZR (A). Appreciable reduction in the interstitial amount of α-SMA in animals treated with benazepril (B), irbesartan (C), and mostly with combined therapy (D). Note in this last group that the vascular wall only presents positive immunostaining (anti–α-SMA, magnification ×400). OZR, obese Zucker rats.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

7. Figure 4
Renal cortex sections with immunostaining for transforming growth factor β1 (TGFβ1) in all groups. (A) shows large area with positive staining in untreated OZR. Considerable decrease in TGFβ1 is observed in animals with benazepril (B) and irbesartan (C), but paramount reduction was appreciated in the combined therapy group (D) (anti-TGFβ1, magnification ×400). OZR, obese Zucker rats.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

8. Figure 4
Renal cortex sections with immunostaining for transforming growth factor β1 (TGFβ1) in all groups. (A) shows large area with positive staining in untreated OZR. Considerable decrease in TGFβ1 is observed in animals with benazepril (B) and irbesartan (C), but paramount reduction was appreciated in the combined therapy group (D) (anti-TGFβ1, magnification ×400). OZR, obese Zucker rats.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

9. Figure 5
Representative images from renal cortex showing localization corresponding to plasminogen activator inhibitor-1 (PAI-1) immunostaining in all groups. Note extensive positive staining in untreated OZR (A), especially in tubular epithelial cells. Lower amount of PAI-1 is observed in treated group as shown in benazepril (B), but especially in irbesartan (C), and almost undetectable in the combined therapy group (D) (anti–PAI-1, magnification ×400). OZR, obese Zucker rats.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

10. Figure 5
Representative images from renal cortex showing localization corresponding to plasminogen activator inhibitor-1 (PAI-1) immunostaining in all groups. Note extensive positive staining in untreated OZR (A), especially in tubular epithelial cells. Lower amount of PAI-1 is observed in treated group as shown in benazepril (B), but especially in irbesartan (C), and almost undetectable in the combined therapy group (D) (anti–PAI-1, magnification ×400). OZR, obese Zucker rats.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions