1. Cryopreservation of human germinal vesicle stage and in vitro matured M II oocytes: influence of cryopreservation media on the survival, fertilization, and early cleavage divisions 
Anuradha Goud, Ph.D., Pravin Goud, M.D., Ph.D., Chen Qian, M.D., Josiane Van der Elst, Ph.D., Georges Van Maele, Ph.D., Marc Dhont, M.D., Ph.D. 
Fertility and Sterility 
Volume 74, Issue 3, Pages (September 2000)
DOI: /S (00)

2. Figure 1
Cryopreservation and thawing at the GV stage frequently resulted in detachment of the cumulus-corona cells from the oocytes, whereas those with the cells attached, often underwent damage. (A), An oocyte that underwent damage with persistently attached cumulus cells (arrow indicates the GV). (B), A surviving oocyte with cumulus cell detachment. (C) and (E), Oocytes that were frozen-thawed either after or before in vitro maturation, respectively, in the low-Na medium. (D), A control GV oocyte showing both persistence and growth of the attached cumulus cells after culture. (F) and (G) are zygotes bearing 2 PN obtained after ICSI on M II oocytes that developed from GV oocytes frozen-thawed in conventional and low-Na media respectively. (H), (I) and (J) are four- to eight-cell embryos that cleaved from in vitro matured oocytes from groups (B), (A), and (C), respectively. All pictures taken under Hoffman contrast at ×200–400 original magnification. Scale bars in D (to represent D), H (to represent H and I) and G (to represent all the rest) correspond to 50 μm.
Goud. Human GV and matured MII oocytes. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )

3. Figure 2
A bar chart representing the rates of cleavage in the experimental and control groups in the context of the zygotes with 2 PN and the total and the surviving GV stage oocytes. Significant differences are indicated as follows: ∗P<.03, †P=.058 (insignificant after Yates’ correction), ‡P<.0001, §P=.003, ∥P=.002, ¶P<.03, and ∗∗P<.04.
Goud. Human GV and matured MII oocytes. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )