1. Volume 31, Issue 4, Pages 557-568 (August 2001)
Activated Notch2 Signaling Inhibits Differentiation of Cerebellar Granule Neuron Precursors by Maintaining Proliferation 
David J. Solecki, XiaoLin Liu, Toshifumi Tomoda, Yin Fang, Mary E. Hatten 
Neuron 
Volume 31, Issue 4, Pages (August 2001)
DOI: /S (01)

2. Figure 1
Genes Required for Transducing the Notch Signaling Cascade Are Expressed in Purified Cerebellar Granule Cells
Cerebellar granule cells were purified (see Experimental Procedures) from postnatal days (P) 5, 6, or 7 mice. Total RNA was isolated. Equal amounts of this RNA were subjected to reverse transcription using random hexamers as a primer for RT and then were amplified by PCR using primers specific for the Notch pathway genes. Amplification of the GAPDH and HPRT messages was used as controls for equal loading. Reactions carried out without reverse transcriptase were used as a control for genomic DNA contamination (data not shown)
Neuron  , DOI: ( /S (01) )

3. Figure 2 Notch2 mRNA Expression in the Developing Cerebellum
Notch2 mRNA is detected in parasagital sections of developing cerebellum of E16 embryos, P0, P6, and P10 mice.
(A) At E16, Notch2 expression is not strong in the cerebellar anlage.
(B) At P0, Notch2 expression in the cerebellum increases and is observable in the Purkinje cell layer and in the EGL that is one to two cells thick at this stage of development.
(C) At P6, Notch2 is expressed highly in the cerebellar EGL and is still observable in the Purkinje cell layer.
(D) At P10, Notch2 expression is reduced in the cerebellum.
(E) A higher magnification of plate (C). Notch2 is highly expressed in proliferating GNPs of the EGL and is downregulated in differentiating granule neurons in deeper aspects of the inner EGL. Notch2 is not expressed in the migrating granule neurons in the molecular layer or postmigratory cells of the internal granule layer. Abbreviations are as follows: rl, rhombic lip; vz, ventricular zone; egl, external granule layer; pcl, Purkinje cell layer; ml, molecular layer; and igl, internal granule layer
Neuron  , DOI: ( /S (01) )

4. Figure 3
Overexpression of the Notch2 ICD Blocks Neurite Extension in Cultured Granule Neurons
Purified P6 cerebellar granule cells cultured as reaggregates extend a halo of neurites when transferred to a poly-L-lysine substrate. GNPs infected with control retrovirus that expresses the AP reporter gene differentiate and possess long neurites within the halo surrounding the reaggregates (A). GNPs overexpressing the Notch2 ICD lack neurites (B). Scale bars equal 50 μm
Neuron  , DOI: ( /S (01) )

5. Figure 4
Overexpression of the Notch2 ICD or HES1 Inhibits the Onset of GNP Differentiation
Morphology and the early differentiation marker TAG-1 was used to assess the differentiation status of infected GNPs.
(A–C) GNPs differentiate normally, extend long neuritis, and express TAG-1 when infected with control retroviruses.
(D–F) A field of four cells overexpressing Notch2 ICD that lack neurites and do not express TAG-1.
(G–I) A field of seven cells overexpressing HES1 that lack neurites and do not express TAG-1. Scale bars equal 20 μm
Neuron  , DOI: ( /S (01) )

6. Figure 5
Quantitation of Neurite Extension and TAG-1 Expression in GNPs Overexpressing the Notch2 ICD or HES1
(A) Two hundred cells were scored for the presence or absence of neurites and were counted from at least three individual experiments. Cells with processes longer than three-cell body lengths were counted as neurite-bearing cells; 81% ± 1.6% of control cells extended neuritis, whereas only 32% ± 6.8% and 28% ± 5.6% of cells overexpressing the Notch2 ICD and HES1 harbored neuritis, respectively.
(B) Neurite negative cells were scored for TAG-1 expression to determine their differentiation status. A cell was considered TAG-1 positive if there was any red signal associated with its cell body; 71% ± 5.3% of the control cells that lacked neuritis expressed TAG-1, indicating that although these cells did not elaborate processes, the majority of these cells had begun differentiation. A lower number of cells overexpressing the Notch2 ICD and HES1 were TAG-1 positive (42% ± 7.5% and 28% ± 5.6%, respectively), indicating that less of these cells had entered the differentiation cascade
Neuron  , DOI: ( /S (01) )

7. Figure 6
Overexpression of the Notch2 ICD and HES1 Inhibits GNP Differentiation In Situ
Organotypic slices cultures prepared from the cerebella of P6 mice were infected with either the control retrovirus expressing GFP or retroviruses expressing the Notch2 ICD or HES1. After a 36 hr of incubation, retrovirally infected cells were visualized by confocal microscopy. A cluster of granule neurons (each individually marked by an arrowhead) infected with the control retrovirus exhibit either the bipolar or t-shaped morphology of differentiated granule neurons (A). Scale Bar equals 50 μm. Parallel fibers are absent from a cluster of two cells overexpressing the Notch2 ICD (B). Scale bar equals 20 μm. Four cells overexpressing HES1 also lack parallel fibers, a phenotype similar to that of the Notch2 ICD overexpressing cells (C). Scale bar equals 20 μm. Note that granule neurons overexpressing the Notch2 ICD or HES1 remain closer to the surface of the cerebellar slice (marked by dashed line) than the control cells. Quantitation of these results revealed that overexpression of either the Notch2 ICD or HES1 reduced the number of cells harboring parallel fibers or t-shaped morphology by two thirds as compared with control cells expressing only GFP (D) (31.6% ± 3.6% or 30.2% ± 5% cells with neuritis, respectively, 93.4% ± 1.9% for the control). The degree of infected cell migration away from the pial surface was quantitated by measuring the location of the cell bodies of 400 infected cells for each virus construct in relation to the pial surface (E). The depths of the measured cell bodies were placed into one of three depth categories: 1–20 μm (black bars), 21–40 μm (white bars), or 41+ μm (gray bars). The majority of cells infected with the control virus were 41+ μm away from the pial surface (60% ± 2%). However, the numbers of cells overexpressing Notch2 ICD or HES1 within this depth category were reduced compared with control (31% ± 4% and 24% ± 3.9%, respectively), a reduction in number of cells that had migrated into deeper regions of the slices
Neuron  , DOI: ( /S (01) )

8. Figure 7 Activated Notch Signaling Maintains GNP Proliferation
(A) Purified postnatal granule cell cultures were incubated with 20 nM Jagged1 or 100 nM Shh. 3H-thymidine was added to the cultures at 48 hr, and incorporation was measured to examine GNP proliferation. Jagged1 stimulates 3H-thymidine incorporation at levels comparable to Shh, indicating that Jagged1 is a mitogen for GNPs.
(B) Organotypic slices cultures prepared from the cerebella of P6 mice were infected with the control, Notch2 ICD, or HES1 retroviruses and were incubated for 30 hr. BrDU was added to the culture and incubated for an additional 8–10 hr to label mitotically active granule neurons. The degree of BrDU incorporation of virally infected cells was then determined by confocal microscopy and was quantitated by the counting of at least 50–100 infected cells per experiment (n = 4). Differentiated GNPs infected with the control retrovirus (each infected cell is marked by arrowheads) exhibit few BrDU-labeled nuclei. Three cells overexpressing the Notch2 ICD lack parallel fibers. Two of these cells appear to be mitotically active, as they possess BrDU-labeled nuclei (marked by elongated arrowheads). A cluster of eight cells overexpressing HES1, each of which possess BrDU-labeled nuclei, is shown
Neuron  , DOI: ( /S (01) )

9. Figure 8 Notch and Shh Signaling Activates Expression of HES1
(A) P6 granule cells were treated with 20 nM Jagged1 or 100 nM ShhN. After 18 hr, total RNA was isolated from the cultures, and the level of HES1 expression was examined by quantitative RT-PCR. Treatment with 20 nM Jagged1 results in a 4.5-fold enhancement in HES1 expression, whereas treatment with 100 nM ShhN results in a 6-fold enhancement.
(B) Cotransfection of a HES1 reporter plasmid with a Shh or Notch2 ICD expression vector leads to potent stimulation of luciferase expression compared with the basal level of expression observed when empty pcDNA3 is cotransfected. Optimal Shh signaling leads to a 28-fold activation, whereas Notch2 ICD activation was roughly three times more potent leading to an 82-fold activation
Neuron  , DOI: ( /S (01) )

10. Figure 9
A Model for Notch2 and HES1 Function in Regulating GNP Differentiation
In this model, GNPs that are competent to differentiate are exposed to multiple signals that regulate their rate of differentiation. Notch2 and Shh mediate mitogenic signals in GNPs, thus preventing the onset of differentiation. Our data suggest that Notch and Shh signaling activates HES1 expression. HES1 maintains GNP proliferation and inhibits differentiation. Therefore, regulation of HES1 expression via these two independent signaling cascades may regulate the balance between proliferation and differentiation of GNPs
Neuron  , DOI: ( /S (01) )