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Volume 87, Issue 2, Pages (August 2004)

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1 Volume 87, Issue 2, Pages 1155-1164 (August 2004)
Porphyrin Depth in Lipid Bilayers as Determined by Iodide and Parallax Fluorescence Quenching Methods and Its Effect on Photosensitizing Efficiency  Irena Bronshtein, Michal Afri, Hana Weitman, Aryeh A. Frimer, Kevin M. Smith, Benjamin Ehrenberg  Biophysical Journal  Volume 87, Issue 2, Pages (August 2004) DOI: /biophysj Copyright © 2004 The Biophysical Society Terms and Conditions

2 Figure 1 Chemical structures of HP3, PP3, and their modified analogs.
Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2004 The Biophysical Society Terms and Conditions

3 Figure 2 Fluorescence spectra [λexc=402nm] of 1μM hematoporphyrin-IX (HP3) in water and in a suspension of lecithin liposomes at increasing concentrations of lipid (in these representative spectra: 0, 15, 37, 55, 113, 212, and 345μg/ml). The arrow indicates the direction of change at increasing lipid concentrations. The intensity of the fluorescence at 626nm is shown in the inset, together with the line, fitted according to Eq. 1. Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2004 The Biophysical Society Terms and Conditions

4 Figure 3 Stern-Volmer quenching plots of the hematoporphyrins (0.3μM) in buffer solutions (full lines) and when bound to lecithin liposomes (0.5mg lipid per ml, dashed lines). Fluorescence intensities were excited at 402nm and measured at 626nm. Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2004 The Biophysical Society Terms and Conditions

5 Figure 4 The distance of the protoporphyrins from the center of the bilayer, obtained by the parallax method, in liposomes composed of DMPC and DOPC. Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2004 The Biophysical Society Terms and Conditions

6 Figure 5 The effect of temperature on the Stern-Volmer iodide-quenching constants of the hematoporphyrins in lecithin liposomes. Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2004 The Biophysical Society Terms and Conditions

7 Figure 6 The effect of temperature on the fluorescence quenching efficiencies of the protoporphyrins by iodide in DMPC liposomes. Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2004 The Biophysical Society Terms and Conditions

8 Figure 7 The effect of temperature on the Stern-Volmer iodide-quenching constants of the hematoporphyrins in DMPC liposomes. Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2004 The Biophysical Society Terms and Conditions


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