1. Expression of Interleukin-12 is Increased in Psoriatic Skin
Nikhil Yawalkar, Stephan Karlen, Robert Hunger, Christoph U. Brand, Lasse R. Braathen 
Journal of Investigative Dermatology 
Volume 111, Issue 6, Pages (December 1998)
DOI: /j x
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Access RT-PCR analysis of IL-12 p35 and p40 expression. Specific transcripts for IL-12 p35 (414 bp) and p40 (373 bp) are shown from normal skin (NS;lanes 1 and 2), nonlesional skin (NL;lanes 3 and 4), and lesional psoriatic skin (LS;lanes 5 and 6). After 45 cycles of amplification the products were analyzed by electrophoresis on 2% agarose and stained by ethidium bromide. M, 100 bp molecular weight marker.Lane 7, negative control with RNA extracted from PBMC containing no AMV-RT in the PCR mix.Lane 8, positive control with PBMC. Amplification of β-actin (838 bp) was used as a control for mRNA integrity.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Detection of IL-12 p35 and p40 transcripts by nested RT-PCR. (A) Optimal number of amplification cycles. RNA samples from normal (NS, n = 5), nonlesional (NL, n = 5), and lesional psoriatic skin (LS, n = 5) were subjected to 25 cycles of RT-PCR and the resulting products to an increasing number of cycles in a nested reaction. The amplified products were electrophoresed on a 2% agarose gel, visualized with ethidium bromide, and analyzed by densitometry. Thex-axis indicates the number of cycles used in the nested reaction and they-axis shows the mean values ± SD of the IL-12 p35 and p40 amplified products measured by densitometry analysis. (B) A representative nested RT-PCR blot from normal (lane 1), nonlesional (lane 2), and psoriatic (lane 3) skin lesions after 25 cycles of amplification for p40 and 27 cycles for p35.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Interleukin-12 p40 mRNA expression is increased in psoriatic lesions. Amplified products of IL-12 p35, p40, and β-actin from normal skin (NS, n = 5), nonlesional skin (NL, n = 5), and lesional psoriatic skin (LS, n = 5) were electrophoresed on 2% agarose gel, visualized with ethidium bromide and analyzed by densitometric scanner. Semi-quantitative analysis was performed using the levels of β-actin transcripts as internal references.y-axes show the relative amount of IL-12 p35 and p40 mRNA.Scale bars: mean ± SD.Asterisk, p < 0.05; comparing paired and unpaired samples using Wilcoxon and Mann–Whitney U test, respectively.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Immunostaining for IL-12 p70 in normal and psoriatic skin. IL-12 immunoreactivity is confined to isolated cells in the dermis of normal skin (a,arrow). Stronger immunoreactivity on mononuclear cells and neutrophils (arrow andinset) was detected in psoriatic lesions (b). No staining was found in the isotype-matched negative control (c). APAAP method,scale bars: 50 μm.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
IL-12 p70 in psoriatic skin is predominantly expressed in the papillary dermis (a) and mainly on mononuclear cells (b). Occasionally, cells with a typical dendritic morphology also showed strong staining for p70 (c).Scale bars: (a) 25 μm; (b, c) 10 μm.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions