1. Corticotropin Releasing Factor Receptor Type 1: Molecular Cloning and Investigation of Alternative Splicing in the Hamster Skin 
Alexander Pisarchik, Andrzej Slominski 
Journal of Investigative Dermatology 
Volume 118, Issue 6, Pages (June 2002)
DOI: /j x
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Alternatively spliced isoforms of CRF-R1. Shadowed boxes = translated exons; open boxes = exons situated after the frameshift. Human and rat (hCRF-R1 and rCRF-R1) sequences are shown to illustrate the differences in gene structure between these species. Numbers above the pictures refer to exon counting. Dashed lines represent sixth human exon, which is absent in rat and hamster CRF-R1 mRNA. Solid lines show the exons that are missing in the described isoform. Arrows indicate position of primers. Double lines represent PCR fragments used for sequencing of hamster CRF-R1 coding sequence. A triangle represents a sequence inserted in hamster CRF-R1h isoform between exons 4 and 5.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Phylogenic analysis of CRF-R1 nucleic acid sequences from different organisms by DNAMLK program (DNA Maximum Likelihood program with molecular clock, version 3.5c, PHYLIP package).
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Alignment of mouse CRF-R1α (accession no. NM_007762), rat CRF-R1A (accession no. L25438), human CRF-R1α (accession no. L23332), and a predicted amino acid sequence of hamster CRF-R1α (GenBank accession no. AY034599). Conserved amino acids are shadowed. Arrows indicate the positions of introns. The putative transmembrane domains are indicated by rows of # symbols below the appropriate amino acids. The numbers in the right-hand column refer to the amino acid number.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Amplification of hamster CRF-R1. The bands are described in Table I. (A)Amplification of fragment spanning exons 2–6: 2, eye; 3, pituitary; 4, heart; 5, skin; 6, melanoma Ma; 7, melanoma MI; 8, melanoma AbC1. (B)Amplification of fragment spanning exons 8–13 (primers P162 and P163): 2, eye; 3, pituitary; 4, heart; 5, spleen; 6, skin; 7, melanoma Ma; 8, melanoma MI; 9, melanoma AbC1. Lane 1 on both pictures is DNA ladder. Arrows indicate sequenced bands.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
Predicted amino acid sequences of hamster CRF-R1 isoforms.Arrows indicate the positions of introns. The putative transmembrane domains are indicated by rows of # symbols below the appropriate amino acid. The numbers in the right-hand column refer to the amino acid number. Predicted amino acid sequences situated after the frameshift are underlined. Dots represent untranslated sequences.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

7. Figure 6
Amplification of hamster CRH-R1 (exons 8–13) in AbC1 cell line. Lines 2 and 5, untreated AbC1 cells; 3, AbC1 cells irradiated by UV (50 mJ per cm2 of UVB); 3, AbC1 cells after induction of melanogenesis. Lines 1 and 4, DNA ladder. Arrows indicate sequenced bands. The bands are described in Table I.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions