Presentation is loading. Please wait.

Presentation is loading. Please wait.

Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps  Tetsuji Takabayashi, MD, Atsushi Kato, PhD,

Published byBora İnci Modified over 5 years ago

Similar presentations

Presentation on theme: "Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps  Tetsuji Takabayashi, MD, Atsushi Kato, PhD,"— Presentation transcript:

1 Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps  Tetsuji Takabayashi, MD, Atsushi Kato, PhD, Anju T. Peters, MD, Lydia A. Suh, BSc, Roderick Carter, BSc, James Norton, MS, Leslie C. Grammer, MD, Bruce K. Tan, MD, Rakesh K. Chandra, MD, David B. Conley, MD, Robert C. Kern, MD, Shigeharu Fujieda, MD, Robert P. Schleimer, PhD  Journal of Allergy and Clinical Immunology  Volume 130, Issue 2, Pages e5 (August 2012) DOI: /j.jaci Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Increased expression of tryptase in NPs. The gene expression of tryptase in UT and NPs (A) and nasal scrapings (B) was measured by using real-time PCR. The concentration of tryptase in tissue homogenates of UT and NPs (C) and nasal lavage (D) was measured by using ELISA. Tryptase concentration was normalized to the concentration of total protein. ∗P < .05, ∗∗P < .001, ∗∗∗P < Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Immunohistochemical detection of tryptase was performed by using antihuman tryptase mAb. Representative immunostaining for tryptase within the epithelium of UT from a control subject (A), a patient with CRSsNP (B), a patient with CRSwNP (C), and an NP (D). Tryptase-positive cells in the glands of UT from a control subject (E) and an NP (F). Magnification ×400. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Quantitation of mast cell protease-positive cells in nasal mucosal tissue from a control subject and patients with CRS and distribution in epithelium, submucosa, and glands. Immunostaining for tryptase (A), chymase (B), and CPA3 (C). ∗P < .05, ∗∗P < .001, ∗∗∗P < Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 The expression of chymase in NPs. The gene expression of chymase in tissue (A) and nasal scrapings (B) was measured by using real-time PCR. Representative immunostaining for chymase within the epithelium of UT from a control subject (C) and an NP (D) and within the glands of UT from a control subject (E) and an NP (F). Magnification ×400. ∗P < .05. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 The expression of CPA3 in NPs. The gene expression of CPA3 in tissue (A) and nasal scrapings (B) was measured by using real-time PCR. Representative immunostaining for CPA3 within the epithelium of UT from a control subject (C) and an NP (D) and within the gland of UT from a control subject (E) and an NP (F). Magnification ×400. ∗∗P < .001, ∗∗∗P < Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig 6 Immunofluorescence of mast cell proteases in NPs. Immunofluorescence assay was performed with antitryptase (green fluorescence) and antichymase (red fluorescence) (A), and anti-CPA3 (red fluorescence) (B) in epithelium and antitryptase (green fluorescence), and antichymase (red fluorescence) (C), and CPA3 (red fluorescence) (D) in glands. Nuclei ware counterstained with 4′,6-diamidino-2-phenylindole (blue fluorescence). The results are representative of 5 separate subjects. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E1 The relationship of tryptase and ECP in UT was evaluated by using ELISA. The correlation was assessed by using the Spearman rank correlation test. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Fig E2 Negative control antibody staining in NPs tissue from a patient with CRSwNP. A, Normal rabbit IgG. B, Anti-human CPA3 antibody. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

10 Fig E3 Correlation of tryptase with candidate mediators for elevation of mast cells. Total RNA was extracted from UT from control subjects (n = 16), patients with CRSsNP (n = 26), patients with CRSwNP (n = 33), and NPs (n = 34). A, The expression of tryptase and candidates for mast cell chemoattractant, SCF, eotaxin-1, eotaxin-2, and eotaxin-3, was analyzed by using real-time PCR. B, The correlations in nasal tissues were assessed by using Spearman rank correlation. ∗∗P < .001, ∗∗∗P < Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps  Tetsuji Takabayashi, MD, Atsushi Kato, PhD,"

Similar presentations

Proton pump inhibitors decrease eotaxin-3/CCL26 expression in patients with chronic rhinosinusitis with nasal polyps: Possible role of the nongastric.

Proton pump inhibitors decrease eotaxin-3/CCL26 expression in patients with chronic rhinosinusitis with nasal polyps: Possible role of the nongastric.
Anju T. Peters, MD, Atsushi Kato, PhD, Ning Zhang, PhD, David B

Anju T. Peters, MD, Atsushi Kato, PhD, Ning Zhang, PhD, David B
Genetic variation in B cell–activating factor of the TNF family (BAFF) and asthma exacerbations among African American subjects  Rajesh Kumar, MD, MS,

Genetic variation in B cell–activating factor of the TNF family (BAFF) and asthma exacerbations among African American subjects  Rajesh Kumar, MD, MS,
Forkhead box protein 3 in human nasal polyp regulatory T cells is regulated by the protein suppressor of cytokine signaling 3  Feng Lan, MD, Nan Zhang,

Forkhead box protein 3 in human nasal polyp regulatory T cells is regulated by the protein suppressor of cytokine signaling 3  Feng Lan, MD, Nan Zhang,
Rubella persistence in epidermal keratinocytes and granuloma M2 macrophages in patients with primary immunodeficiencies  Ludmila Perelygina, PhD, Stanley.

Rubella persistence in epidermal keratinocytes and granuloma M2 macrophages in patients with primary immunodeficiencies  Ludmila Perelygina, PhD, Stanley.
Striking deposition of toxic eosinophil major basic protein in mucus: Implications for chronic rhinosinusitis  Jens U. Ponikau, MD, David A. Sherris,

Striking deposition of toxic eosinophil major basic protein in mucus: Implications for chronic rhinosinusitis  Jens U. Ponikau, MD, David A. Sherris,
Russell S. Traister, MD, PhD, Crystal E. Uvalle, BS, Gregory A

Russell S. Traister, MD, PhD, Crystal E. Uvalle, BS, Gregory A
Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.

Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.
Forkhead box protein 3 in human nasal polyp regulatory T cells is regulated by the protein suppressor of cytokine signaling 3  Feng Lan, MD, Nan Zhang,

Forkhead box protein 3 in human nasal polyp regulatory T cells is regulated by the protein suppressor of cytokine signaling 3  Feng Lan, MD, Nan Zhang,
Proton pump inhibitors decrease eotaxin-3/CCL26 expression in patients with chronic rhinosinusitis with nasal polyps: Possible role of the nongastric.

Proton pump inhibitors decrease eotaxin-3/CCL26 expression in patients with chronic rhinosinusitis with nasal polyps: Possible role of the nongastric.
The antimicrobial protein short palate, lung, and nasal epithelium clone 1 (SPLUNC1) is differentially modulated in eosinophilic and noneosinophilic chronic.

The antimicrobial protein short palate, lung, and nasal epithelium clone 1 (SPLUNC1) is differentially modulated in eosinophilic and noneosinophilic chronic.
Heterogeneous inflammatory patterns in chronic rhinosinusitis without nasal polyps in Chicago, Illinois  Bruce K. Tan, MD, MS, Aiko I. Klingler, PhD,

Heterogeneous inflammatory patterns in chronic rhinosinusitis without nasal polyps in Chicago, Illinois  Bruce K. Tan, MD, MS, Aiko I. Klingler, PhD,
Ryan H. Dougherty, MD, Sukhvinder S

Ryan H. Dougherty, MD, Sukhvinder S
Proprotein convertases generate a highly functional heterodimeric form of thymic stromal lymphopoietin in humans  Julie A. Poposki, MS, Aiko I. Klingler,

Proprotein convertases generate a highly functional heterodimeric form of thymic stromal lymphopoietin in humans  Julie A. Poposki, MS, Aiko I. Klingler,
Evidence for intranasal antinuclear autoantibodies in patients with chronic rhinosinusitis with nasal polyps  Bruce K. Tan, MD, Quan-Zhen Li, PhD, Lydia.

Evidence for intranasal antinuclear autoantibodies in patients with chronic rhinosinusitis with nasal polyps  Bruce K. Tan, MD, Quan-Zhen Li, PhD, Lydia.
IL-25 as a novel therapeutic target in nasal polyps of patients with chronic rhinosinusitis  Hyun-Woo Shin, MD, PhD, Dong-Kyu Kim, MD, Min-Hyun Park, MD,

IL-25 as a novel therapeutic target in nasal polyps of patients with chronic rhinosinusitis  Hyun-Woo Shin, MD, PhD, Dong-Kyu Kim, MD, Min-Hyun Park, MD,
Anju T. Peters, MD, Atsushi Kato, PhD, Ning Zhang, PhD, David B

Anju T. Peters, MD, Atsushi Kato, PhD, Ning Zhang, PhD, David B
Extracellular eosinophilic traps in association with Staphylococcus aureus at the site of epithelial barrier defects in patients with severe airway inflammation 

Extracellular eosinophilic traps in association with Staphylococcus aureus at the site of epithelial barrier defects in patients with severe airway inflammation 
Expression of 11β-hydroxysteroid dehydrogenase 1 and 2 in patients with chronic rhinosinusitis and their possible contribution to local glucocorticoid.

Expression of 11β-hydroxysteroid dehydrogenase 1 and 2 in patients with chronic rhinosinusitis and their possible contribution to local glucocorticoid.
Evidence of a role for B cell–activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps  Atsushi Kato, PhD,

Evidence of a role for B cell–activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps  Atsushi Kato, PhD,

Similar presentations

Ads by Google