Presentation is loading. Please wait.

Presentation is loading. Please wait.

Mast Cell–Fibroblast Interactions: Human Mast Cells as Source and Inducers of Fibroblast and Epithelial Growth Factors  Metin Artuc, U. Muscha Steckelings,

Published byAygül Özgen Modified over 5 years ago

Similar presentations

Presentation on theme: "Mast Cell–Fibroblast Interactions: Human Mast Cells as Source and Inducers of Fibroblast and Epithelial Growth Factors  Metin Artuc, U. Muscha Steckelings,"— Presentation transcript:

1 Mast Cell–Fibroblast Interactions: Human Mast Cells as Source and Inducers of Fibroblast and Epithelial Growth Factors  Metin Artuc, U. Muscha Steckelings, Beate M. Henz  Journal of Investigative Dermatology  Volume 118, Issue 3, Pages (March 2002) DOI: /j x x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Human dermal mast cells express various growth factors. Representative agarose gel showing amplification products of mRNA encoding FGF-2, FGF-7, FGF-10, and HB-EGF. No positive signals were noted for FGF-5 and HGF. Journal of Investigative Dermatology  , DOI: ( /j x x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Human dermal mast cells express FGF-2 protein. FGF-2 was detected in cell supernatants as well as in homogenates by ELISA. FGF-2 could not be further augmented by stimulation with IL-4 (5 ng per ml) or SCF (50 ng per ml) or a combination of both. Data are given as mean ± SEM, n = 3. Journal of Investigative Dermatology  , DOI: ( /j x x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 HMC-1 cells express FGF-2 protein, and FGF-2 expression is enhanced by PMA. FGF-2 was detected in cell supernatants by ELISA. A low basal secretion of FGF-2 protein could be markedly and dose-dependently enhanced by stimulation with PMA, reaching a maximum at 10 µg per ml PMA added. Data are given as mean ± SEM, n = 3. Journal of Investigative Dermatology  , DOI: ( /j x x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Expression of FGF-7 protein by human dermal fibroblasts can be stimulated by mast cell supernatants. FGF-7 was detected in fibroblast supernatants by ELISA: (A) controls; (B) fibroblasts stimulated with PMA; (C) fibroblasts stimulated with supernatants derived from unstimulated HMC-1 cells; (D) fibroblasts stimulated with supernatants derived from HMC-1 cells stimulated with PMA (40 µg per ml). Data are given as mean ± SEM, n = 3. Journal of Investigative Dermatology  , DOI: ( /j x x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Tryptase stimulates FGF-2 secretion by human dermal fibroblasts. FGF-2 and FGF-7 were detected in fibroblast supernatants by ELISA. Tryptase (0.05–5 mU) stimulated FGF-2 secretion by human dermal fibroblasts with a maximum at 0.5 mU, but had no effect on FGF-7 secretion. Data are given as mean ± SEM (FGF-7, n = 3; FGF-2, n = 5). Statistical analyses were performed using Student's two-tailed t test. *p < 0.05 vs control; **p < vs control. Journal of Investigative Dermatology  , DOI: ( /j x x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 Histamine stimulates FGF-7 secretion by human dermal fibroblasts. FGF-2 and FGF-7 were detected in fibroblast supernatants by ELISA. Histamine (10−4-10−9 M) dose-dependently stimulated FGF-7 secretion, with a maximum at 10−5 M, but had no effect on FGF-2 secretion. Data are given as mean ± SEM, n = 3. Journal of Investigative Dermatology  , DOI: ( /j x x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

8 Figure 7 Histamine-induced stimulation of FGF-7 secretion by human dermal fibroblasts is mediated via H1 receptors. The histamine-induced stimulation of FGF-7 secretion (10−6 M) could be completely blocked by preincubation with the H1-histamine-receptor antagonist descarboethoxyloratadine (10−6-10−8 M), whereas preincubation with the specific H2-histamine-receptor antagonist famotidin (10−6-10−8 M) had no effect. Data are given as mean ± SEM, n = 3. Journal of Investigative Dermatology  , DOI: ( /j x x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Mast Cell–Fibroblast Interactions: Human Mast Cells as Source and Inducers of Fibroblast and Epithelial Growth Factors  Metin Artuc, U. Muscha Steckelings,"

Similar presentations

Glucocorticoids Augment the Chemically Induced Production and Gene Expression of Interleukin-1α through NF-κB and AP-1 Activation in Murine Epidermal.

Glucocorticoids Augment the Chemically Induced Production and Gene Expression of Interleukin-1α through NF-κB and AP-1 Activation in Murine Epidermal.
IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,
Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal.

Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal.
Keloid Fibroblasts Resist Ceramide-Induced Apoptosis by Overexpression of Insulin- Like Growth Factor I Receptor  Hiroshi Ishihara, Hiroshi Yoshimoto,

Keloid Fibroblasts Resist Ceramide-Induced Apoptosis by Overexpression of Insulin- Like Growth Factor I Receptor  Hiroshi Ishihara, Hiroshi Yoshimoto,
Histamine Induces Melanogenesis and Morphologic Changes by Protein Kinase A Activation via H2 Receptors in Human Normal Melanocytes  Masaki Yoshida, Yoshito.

Histamine Induces Melanogenesis and Morphologic Changes by Protein Kinase A Activation via H2 Receptors in Human Normal Melanocytes  Masaki Yoshida, Yoshito.
Epidermal Growth Factor Induces Fibronectin Expression in Human Dermal Fibroblasts via Protein Kinase C δ Signaling Pathway  Yoshihiro Mimura, Hironobu.

Epidermal Growth Factor Induces Fibronectin Expression in Human Dermal Fibroblasts via Protein Kinase C δ Signaling Pathway  Yoshihiro Mimura, Hironobu.
Insulin-Like Growth Factor-I Enhances Transforming Growth Factor-β-Induced Extracellular Matrix Protein Production Through the P38/Activating Transcription.

Insulin-Like Growth Factor-I Enhances Transforming Growth Factor-β-Induced Extracellular Matrix Protein Production Through the P38/Activating Transcription.
Keratinocyte Growth Regulation in Defined Organotypic Cultures Through IL-1-Induced Keratinocyte Growth Factor Expression in Resting Fibroblasts  Nicole.

Keratinocyte Growth Regulation in Defined Organotypic Cultures Through IL-1-Induced Keratinocyte Growth Factor Expression in Resting Fibroblasts  Nicole.
Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes  Marcel B.M. Teunissen, Jan.

Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes  Marcel B.M. Teunissen, Jan.
Regulation of HMG-CoA Synthase and HMG-CoA Reductase by Insulin and Epidermal Growth Factor in HaCaT Keratinocytes  Ian R. Harris, Hendrik Höppner, Wilfried.

Regulation of HMG-CoA Synthase and HMG-CoA Reductase by Insulin and Epidermal Growth Factor in HaCaT Keratinocytes  Ian R. Harris, Hendrik Höppner, Wilfried.
A Fibrogenic Cytokine, Platelet-Derived Growth Factor (PDGF), Enhances Mast Cell Growth Indirectly Via a SCF- and Fibroblast-Dependent Pathway  Takaaki.

A Fibrogenic Cytokine, Platelet-Derived Growth Factor (PDGF), Enhances Mast Cell Growth Indirectly Via a SCF- and Fibroblast-Dependent Pathway  Takaaki.
Pseudomonas Aeruginosa- and IL-1β-Mediated Induction of Human β-Defensin-2 in Keratinocytes Is Controlled by NF-κB and AP-1  Kai Wehkamp, Lars Schwichtenberg,

Pseudomonas Aeruginosa- and IL-1β-Mediated Induction of Human β-Defensin-2 in Keratinocytes Is Controlled by NF-κB and AP-1  Kai Wehkamp, Lars Schwichtenberg,
Minoxidil-Induced Hair Growth is Mediated by Adenosine in Cultured Dermal Papilla Cells: Possible Involvement of Sulfonylurea Receptor 2B as a Target.

Minoxidil-Induced Hair Growth is Mediated by Adenosine in Cultured Dermal Papilla Cells: Possible Involvement of Sulfonylurea Receptor 2B as a Target.
Histamine Contributes to Tissue Remodeling via Periostin Expression

Histamine Contributes to Tissue Remodeling via Periostin Expression
17β-estradiol, Progesterone, and Dihydrotestosterone Suppress the Growth of Human Melanoma by Inhibiting Interleukin-8 Production  Naoko Kanda, Shinichi.

17β-estradiol, Progesterone, and Dihydrotestosterone Suppress the Growth of Human Melanoma by Inhibiting Interleukin-8 Production  Naoko Kanda, Shinichi.
IGF-II-Mediated COX-2 Gene Expression in Human Keratinocytes Through Extracellular Signal-Regulated Kinase Pathway  Hye Jung Kim, Tae-Yoon Kim  Journal.

IGF-II-Mediated COX-2 Gene Expression in Human Keratinocytes Through Extracellular Signal-Regulated Kinase Pathway  Hye Jung Kim, Tae-Yoon Kim  Journal.
Maksim V. Plikus  Journal of Investigative Dermatology 

Maksim V. Plikus  Journal of Investigative Dermatology 
Kavitha K. Reddy  Journal of Investigative Dermatology 

Kavitha K. Reddy  Journal of Investigative Dermatology 
Ultraviolet Light and Interleukin-10 Modulate Expression of Cytokines by Transformed Human Dermal Microvascular Endothelial Cells (HMEC-1)  Thomas Scholzen,

Ultraviolet Light and Interleukin-10 Modulate Expression of Cytokines by Transformed Human Dermal Microvascular Endothelial Cells (HMEC-1)  Thomas Scholzen,
17β-Estradiol Enhances Vascular Endothelial Growth Factor Production and Dihydrotestosterone Antagonizes the Enhancement via the Regulation of Adenylate.

17β-Estradiol Enhances Vascular Endothelial Growth Factor Production and Dihydrotestosterone Antagonizes the Enhancement via the Regulation of Adenylate.

Similar presentations

Ads by Google