1. EGFL7 increased surface expression of integrins α5β1 and αVβ3
EGFL7 increased surface expression of integrins α5β1 and αVβ3 ASchematic of the experimental setup to test for surface expression of integrins α5β1 and αVβ3 in human umbilical vein endothelial cells (HUVEC). All surface molecules were marked by biotinylation followed by immunoprecipitation (IP) and Western blot specifically detecting each integrin α or β subunit. Cells have not been permeabilized in either case to avoid staining of intracellular proteins. Alternatively, antibodies specific for both integrins were applied and detected by FACS.B–DSurface biotinylation assays verified increased surface expression of αV and β3 upon application of E7 but not Fn (n = 3; one‐way ANOVA).E–GSurface expression of α5 and β1 was found increased upon treatment with E7 or Fn and was further enhanced by combination of both (n = 3; one‐way ANOVA).HEctopic expression of murine EGFL7 (mE7) in GL261 mouse glioma cells followed by intracranial implantation into the striatum of immune‐competent mice reduced overall survival but was rescued upon treatment with an integrin α5β1‐inhibiting antibody (n = 6; log‐rank test).Data information: Data presented as mean ± SEM. TCL, total cell lysate; AU, arbitrary units.Source data are available online for this figure.
Nevenka Dudvarski Stanković et al. EMBO Mol Med. 2018;10:e8420
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