1. Department of Computer Science Ben-Gurion University
Biological Application: RNAs
Research Focus: Searching for Novel Riboswitches in Newly Sequenced Genomes
Danny Barash†
Department of Computer Science
Ben-Gurion University †
Research focus on riboswitches
Bioinformatics

2. What are Riboswitches? Vitamin B1 biosynthesis in bacteria:
With TPP
Terminator
Vitamin B1 biosynthesis in bacteria:
TPP-mediated attentuation control:
E. Nudler and coworkers:
(Mironov et al., Cell, 2002)
Thiamine pyrophosphate are the metabolites that alter conformation.
Aptamer
Research focus on riboswitches
Bioinformatics

3. What are Riboswitches? Vitamin B1 biosynthesis in bacteria:
Without TPP
Vitamin B1 biosynthesis in bacteria:
TPP-mediated attentuation control:
E. Nudler and coworkers:
(Mironov et al., Cell, 2002)
Thiamine pyrophosphate are the metabolites that alter conformation.
Anti-terminator
Aptamer
Research focus on riboswitches
Bioinformatics

4. Searching for riboswitches in eukaryotes by their aptamer
Sequence is not enough, need structure considerations!
Research focus on riboswitches
Bioinformatics

5. Searching for riboswitches in eukaryotes by their aptamer
(Cohen et al., In Silico Biology, 2008) – a riboswitch in plants?
Known consensus
in bacteria
Our 3-stem junction
candidate in plants
Research focus on riboswitches
Bioinformatics