1. Mercury uptake in Shewanella Oneidensis
Alex Szczuka Adviser: Dr. Jeffra Schaefer
Prof. Francois Morel Lab

2. Mercury Cycle
Methylmercury = toxic = accumulation

3. S. Oneidensis: a non-methylator
Same environment as methylators
Aerobic and anaerobic
Is the uptake the same in the presence and lack of oxygen?

4. Procedure Harvest in exponential growth phase Set up conditions
Wash cells
Measure mercury content

5. Mercury uptake is active
Uptake with CCCP shown by connected dots;Uptake in the presence of Cysteine disconnected

6. Uptake could depend on oxygen

7. Summary
Mercury uptake is active
Uptake could depend on oxygen

8. Thank You!
Dr. Jeffra Schaefer
Prof. Francois Morel
PEI

9. Image references