1. Morphokinetic analysis and embryonic prediction for blastocyst formation through an integrated time-lapse system 
Yamileth Motato, Ph.D., María José de los Santos, Ph.D., María José Escriba, Ph.D., Belén Aparicio Ruiz, Ph.D., José Remohí, M.D., Marcos Meseguer, Ph.D. 
Fertility and Sterility 
Volume 105, Issue 2, Pages e9 (February 2016)
DOI: /j.fertnstert
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Hierarchical classification of embryos based on [1] timing of morula, optimal range 81.28–96.00 hours; [2] timing of transition from 5-blastomere embryo to until 8-blastomere embryo (t8–t5), optimal range ≤8.78 hours. The classification generates four categories of embryos with increasing expected blastocyst formation (right to left).
Fertility and Sterility  , e9DOI: ( /j.fertnstert )
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3. Figure 2
Implantation rate of embryos exhibiting or not optimal ranges for tEB and t8–t5 kinetic parameters. Time ranges are described in hours after ICSI. *P<.05.
Fertility and Sterility  , e9DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

4. Supplemental Figure 1
Flowchart for phases accomplished in this study to develop the model.
Fertility and Sterility  , e9DOI: ( /j.fertnstert )
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5. Supplemental Figure 2
Blastocyst morphology classification categories based on trophectoderm, inner cell mass, and grade of expansion.
Fertility and Sterility  , e9DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

6. Supplemental Figure 3
Graphic description of morphokinetic variables discerned by time-lapse analysis.
Fertility and Sterility  , e9DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

7. Supplemental Figure 4
Hierarchical classification of embryos based on morphological screening; the new morphological criteria and timing of cell divisions based on optimal range for t5 or timing of cell division to 5 cells (48.8–56.6 hours); optimal range for second synchrony t4–t3 (≤0.76 hours); and optimal range for t3–t2 or duration of second cell cycle (≤11.9 hours). This classification was applied for embryo selection as described by Meseguer et al. (13).
Fertility and Sterility  , e9DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions