Presentation is loading. Please wait.

Presentation is loading. Please wait.

Ex vivo gene delivery of ephrin-B2 induces development of functional collateral vessels in a rabbit model of hind limb ischemia  Masatake Katsu, MD, Hiroyuki.

Published bySabina Dennis Modified over 5 years ago

Similar presentations

Presentation on theme: "Ex vivo gene delivery of ephrin-B2 induces development of functional collateral vessels in a rabbit model of hind limb ischemia  Masatake Katsu, MD, Hiroyuki."— Presentation transcript:

1 Ex vivo gene delivery of ephrin-B2 induces development of functional collateral vessels in a rabbit model of hind limb ischemia  Masatake Katsu, MD, Hiroyuki Koyama, MD, Hiromitsu Maekawa, MD, Hiroki Kurihara, MD, Hiroaki Uchida, MD, Hirohumi Hamada, MD  Journal of Vascular Surgery  Volume 49, Issue 1, Pages (January 2009) DOI: /j.jvs Copyright © 2009 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 A, Western blot analysis showed in vitro expression of ephrin-B2 on AxCAmephrinB2-treated rabbit fibroblasts. Ephrin-B2 was detected at 43 kD. B, Western blot analysis of Eph-B4 showed in vitro expression of Eph-B4 (about 130 kD) on AxCAmEphB4-treated fibroblasts. C, Photographs showed a mixture of (left panel) AxCAmephrinB2-treated fibroblasts and AxCAmEphB4-treated fibroblasts and (right panel) a mixture of AxCAmephrinB2-treated fibroblasts and AxCALacZ-treated fibroblasts. Obvious aggregation of cells is observed in the left panel, but no aggregation is observed in the right panel. C, Control sample (lysate of cells not infected); d, days after infection. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Calf blood pressure ratio (ratio of left calf systolic pressure to right calf systolic pressure) was measured immediately after femoral artery removal (flow was not detected) and immediately before cell administration in control group (open circles, n = 12) vs ephrin group (filled circles, n = 10), and 28 days after cell administration. Mean values are shown with the standard deviation (range bars). *P < NS, No significant difference. P = Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 A, Selective internal iliac angiograms from animals in control group and (B) ephrin group at 28 days after cell administration. C, Development of collateral vessels was quantified by angiographic score in the control group (white bar, n = 12) vs the ephrin group (filled bar, n = 10). Mean values are shown with the standard deviation (range bars). *P = Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 A, In vivo blood flow of left internal iliac artery was measured with a Doppler guidewire. Average peak velocity was recorded at rest in the control group (white bar, n = 11) and ephrin group (filled bar, n = 10), maximum average peak velocity was measured after papaverine injection, and blood flow was calculated. B, Collateral conductance representing the function of developed collateral vessels in the control group and ephrin group. Mean values are presented with the standard deviation (range bars). *P = .0014; **P = .0007; ***P = Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 The Society for Vascular Surgery Terms and Conditions

6 Fig 5 A, Alkaline phosphatase staining of ischemic hind limb muscle in control group and (B) ephrin group. Panels A and B magnification × 400. C, Capillary density of left semimembranous muscle was measured in tissue sections stained by indoxyl-tetrazolium method in control group (white bar, n = 12) and ephrin group (filled bar, n = 10). Mean values are presented with the standard deviation (range bars). P = Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 The Society for Vascular Surgery Terms and Conditions

Download ppt "Ex vivo gene delivery of ephrin-B2 induces development of functional collateral vessels in a rabbit model of hind limb ischemia  Masatake Katsu, MD, Hiroyuki."

Similar presentations

Enhanced angiogenesis and growth of collaterals by in vivo administration of recombinant basic fibroblast growth factor in a rabbit model of acute lower.

Enhanced angiogenesis and growth of collaterals by in vivo administration of recombinant basic fibroblast growth factor in a rabbit model of acute lower.
Vascular endothelial growth factor-C derived from CD11b+ cells induces therapeutic improvements in a murine model of hind limb ischemia  Go Kuwahara,

Vascular endothelial growth factor-C derived from CD11b+ cells induces therapeutic improvements in a murine model of hind limb ischemia  Go Kuwahara,
Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.
Angiogenic properties of sustained release platelet-rich plasma: Characterization in-vitro and in the ischemic hind limb of the mouse  Shyamal Chandra.

Angiogenic properties of sustained release platelet-rich plasma: Characterization in-vitro and in the ischemic hind limb of the mouse  Shyamal Chandra.
High mobility group box 1 promotes endothelial cell angiogenic behavior in vitro and improves muscle perfusion in vivo in response to ischemic injury 

High mobility group box 1 promotes endothelial cell angiogenic behavior in vitro and improves muscle perfusion in vivo in response to ischemic injury 
Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.
Therapeutic angiogenesis using autologous bone marrow stromal cells: improved blood flow in a chronic limb ischemia model  Abdulaziz Al-Khaldi, MD, Hilal.

Therapeutic angiogenesis using autologous bone marrow stromal cells: improved blood flow in a chronic limb ischemia model  Abdulaziz Al-Khaldi, MD, Hilal.
In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,

In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,
Ulka Sachdev, MD, Xiangdong Cui, MS, Edith Tzeng, MD 

Ulka Sachdev, MD, Xiangdong Cui, MS, Edith Tzeng, MD 
Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis  April E. Nedeau, MD,

Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis  April E. Nedeau, MD,
The therapeutic effect of vascular endothelial growth factor gene- or heme oxygenase-1 gene-modified endothelial progenitor cells on neovascularization.

The therapeutic effect of vascular endothelial growth factor gene- or heme oxygenase-1 gene-modified endothelial progenitor cells on neovascularization.
Direct Comparison of Umbilical Cord Blood versus Bone Marrow–Derived Endothelial Precursor Cells in Mediating Neovascularization in Response to Vascular.

Direct Comparison of Umbilical Cord Blood versus Bone Marrow–Derived Endothelial Precursor Cells in Mediating Neovascularization in Response to Vascular.
An engineered vascular endothelial growth factor-activating transcription factor induces therapeutic angiogenesis in ApoE knockout mice with hindlimb.

An engineered vascular endothelial growth factor-activating transcription factor induces therapeutic angiogenesis in ApoE knockout mice with hindlimb.
The bone marrow-derived endothelial progenitor cell response is impaired in delayed wound healing from ischemia  Stephen M. Bauer, MD, Lee J. Goldstein,

The bone marrow-derived endothelial progenitor cell response is impaired in delayed wound healing from ischemia  Stephen M. Bauer, MD, Lee J. Goldstein,
Hypertensive extracorporeal limb perfusion (HELP): A new technique for managing critical lower limb ischemia  Rodney J. Lane, MD, Mark Phillips, PhD,

Hypertensive extracorporeal limb perfusion (HELP): A new technique for managing critical lower limb ischemia  Rodney J. Lane, MD, Mark Phillips, PhD,
A patient with multiple paradoxical emboli

A patient with multiple paradoxical emboli
Hybrid Surgical Angiogenesis: Omentopexy Can Enhance Myocardial Angiogenesis Induced by Cell Therapy  Taro Kanamori, MD, Go Watanabe, MD, Tamotsu Yasuda,

Hybrid Surgical Angiogenesis: Omentopexy Can Enhance Myocardial Angiogenesis Induced by Cell Therapy  Taro Kanamori, MD, Go Watanabe, MD, Tamotsu Yasuda,
Elaine Y. L. Lui, MSc, Aaron H. Steinman, PhD, Richard S. C

Elaine Y. L. Lui, MSc, Aaron H. Steinman, PhD, Richard S. C
Gale Tang, MD, David N. Charo, Rong Wang, PhD, Israel F

Gale Tang, MD, David N. Charo, Rong Wang, PhD, Israel F
VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery  Yanming Qu, MD, Xiangen Shi, MD, Hongwei Zhang,

VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery  Yanming Qu, MD, Xiangen Shi, MD, Hongwei Zhang,

Similar presentations

Ads by Google