1. Inhibition of Melanosome Transfer Results in Skin Lightening1
Miri Seiberg, Christine Paine, Elizabeth Sharlow, Magdalena Eisinger, Stanley S. Shapiro 
Journal of Investigative Dermatology 
Volume 115, Issue 2, Pages (August 2000)
DOI: /j x
Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
The effect of RWJ and SLIGRL on pigmentation in individual melanocytes. Equivalents were treated with SLIGRL (10 μM) and with RWJ (0.1 μM) for 3 d, followed by F&M staining of histologic sections. Melanocytes were from a Hispanic donor. Images of individual melanocytes are shown. Left panels, untreated control; middle panels, RWJ-50353; right panels, SLIGRL. Scale bar: 10 μM.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
The PAR-2 pathway affects melanosome ingestion by keratinocytes. Melanosomes were isolated from Melan-A cells according toOrlow et al. (1994). Images of HaCaT keratinocytes untreated (a) or treated with SLIGRL (10 μM, (b)) or RWJ (10 μM, (c)) for 2 d, followed by a 2h incubation with the isolated melanosomes, extensive wash, and F&M staining. Scale bar: 10 μM. (d) Melanin area per cell, quantified by image analysis.
Journal of Investigative Dermatology  , DOI: ( /j x)
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4. Figure 3
Electron microscopy analysis of the RWJ effect on equivalents. Representative melanosomes as identified in (a) control and (b) RWJ (100 μM) treated equivalents. (c) A melanocyte dendrite, containing melanosomes, inside an RWJ treated keratinocyte. Such structures could not be easily identified in untreated controls. Scale bar: (a, b) 0.1 μM; (c) 0.5 μM.
Journal of Investigative Dermatology  , DOI: ( /j x)
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5. Figure 4
RWJ induced depigmentation in vivo. Yucatan swine were treated with vehicle (a), and 10 μM (b), 50 μM (c), and 250 μM (d) of RWJ for 8 wk. (a) Picture of the swine (both sides) after 8 wk of treatment. (b) Chromameter measurements of skin color (L* scale, 0=black, 100=white) during the treatment phase. Both a dose response and a time response are observed.
Journal of Investigative Dermatology  , DOI: ( /j x)
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6. Figure 5
Histologic analysis of swine skin samples treated with RWJ Swine were treated as described in Figure 4 and biopsies were taken at the eighth week of treatment. F&M staining of skin sections revealed a dose-dependent reduction in melanin deposition at treated sites: (a) vehicle; (b)–(d) 10 μM, 50 μM, and 250 μM of RWJ Scale bar:20 μM. (e) Relative pigmentation, calculated as melanin area per epidermis area and normalized to untreated controls, obtained by image analysis of F&M-stained skin sections.
Journal of Investigative Dermatology  , DOI: ( /j x)
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7. Figure 6
The depigmenting effect of RWJ is reversible. Yucatan swine were treated for 8 wk with 250 μM of RWJ-50353, and followed without treatment for a further 4 wk. Biopsies were taken before the start of treatment (a), after 8 wk (completion of treatment phase, b), and at the ninth, tenth, and twelfth weeks (c–e, 1–4 wk after treatment was terminated). Scale bar: 12 μM. F&M-stained sections revealed re-pigmentation after treatment had been stopped, with no irritation or other side-effects. Differences in epidermal thickness result from the different sites of biopsies, and have no correlation to treatments. (f) Image analysis of F&M-stained sections was used to quantify pigmentation, relative to the untreated control.
Journal of Investigative Dermatology  , DOI: ( /j x)
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8. Figure 7
Electron micrpscopy analysis of Yucatan swine skin treated with RWJ (a) A representative melanosome inside a keratinocyte of untreated swine skin. (b), (c) Representative melanosomes inside keratinocytes of RWJ (250 μM) treated swine skin are smaller and less pigmented than control ones. (d) A random distribution of melanosomes in control swine epidermis. (e) Melanosomes of RWJ (250 μM) treated swine skin are detected mainly at the epidermal–dermal border (marked). Scale bar: (a-c) 0.05 μM; (d, e) 0.8 μM.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions