1. Mechanical Bioreactor
Build and Test Prep
Amanda Castagnino
Emily Adams
Emily Wood
Natalie Nold
Simran Singh

2. Agenda Goals and Deliverables Updated Cell Chamber Design
Updated Device Model Design
Electrical Schematic
Cell Analysis Update
Problem-Tracking Document
Risk Assessment Update
Bill of Materials Update
MSD II Project Plan
Agenda

3. PHASE I GOALS PHASE I DELIVERABLES
Create electrical schematics for device
Finalize CAD model of device
Create new iteration of chamber mold
PHASE I DELIVERABLES
Updated PDMS mold of cell chamber.
Electrical Schematic
Complete CAD model of device
Problem-Tracking Document
Updated Risk Assessment
Updated Bill of Materials
MSD II Project Plan

4. Chamber Mold
I will say something along these lines: The main change we made to this mold compared to the others is that there will be a cut out on either side of the top mold in between the pegs. This allows for a viewing window for the user to be able to see how much PDMS is in the mold. It will also act as the “breather holes” to allow air to escape during the curing process.

5. CAD Models

7. CAD Models

8. CAD Models

9. Electrical Schematic

10. Electrical Schematic (cont.)
Arduino/LCD screen

11. Motor Controller

12. Cell Analysis Update
Discussed with Dr. Abhyankar about how to use ImageJ to analysis cells for experiment
Changes in cell orientation can be analyzed
Changes in cell size (length and width) can be analyze
There are plugins that Dr. Abhyankar is developing in his lab to do some similar cell analysis that we may be able to use

13. Risk Assessment
Added risk for loss of function due to difficult user interface. This arose from decision to forgo Labview user interface. This risk may be lowered by having a LED screen user interface rather than making the student use Arduino code.

14. Boxed: Items recently ordered.
Bill of Materials
Boxed: Items recently ordered.
Boxed items were items purchased this phase. New electronic items will be purchased once actuator movement is achieved?

15. Problem-Tracking
Discuss Mike’s expectations for this document, and does it need to be on EDGE this phase?

16. Develop working prototype
MSD II Project Plan
Next phase:
Develop working prototype
3-D print model
Assemble prototype device
Achieve device movement
Strength testing for heat cured PDMS (once vacuum oven is functional)
Imagine RIT Submission and final report draft

17. MSD II Project Plan Static cell culture testing and ImageJ analysis
Define user input for device movement
Test experiment conditions and ease of use
Static cell culture testing and ImageJ analysis
Dynamic cell culture testing with working device
Does the team feel this is realistic for cell culture testing?