1. Immunolabeling and Biochemical Analysis of Pectin DM in Seeds and Mucilage.(A) to (D) 2F4 immunolabeling (green) of unesterified HG and seed intrinsic fluorescence (magenta).(A) and (B) Maximum intensity signals from multiple optical stacks.(C) and (D) Opti...
Immunolabeling and Biochemical Analysis of Pectin DM in Seeds and Mucilage.(A) to (D) 2F4 immunolabeling (green) of unesterified HG and seed intrinsic fluorescence (magenta).(A) and (B) Maximum intensity signals from multiple optical stacks.(C) and (D) Optical slices through the middle of seeds. In the wild type (WT), 2F4 labels attached primary walls (arrowheads). For fly1-1, 2F4 labels both the detached primary walls (arrows) and the underlying mucilage. Bars = 50 µm.(F) Biochemical determination of pectin DM in mucilage and whole seeds. Wild-type and fly1-1 whole seeds have a similar DM, but fly1-1 mucilage has a significantly lower DM than wild-type mucilage. Values represent the mean ± se of four technical replicates. Two additional biological replicates showed the same trend.
Cătălin Voiniciuc et al. Plant Cell 2013;25:
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