1. Measurement & Micropipetting

2. Metric conversions
Will be measuring extremely small amounts of liquids – less than a drop!
Units we use are milliliters, mL, (0.001 of a liter) and microliters, μL, (0.001 of a milliliter)

3. Metric conversions
One Liter
is almost
equal to
one quart
(32 oz.)

4. Using Micropipettors
$185

5. Using Micropipettors Never:
Rotate volume beyond the upper or lower range of the pipet
Use without a tip.
Lay a filled pipettor down

6. Using Micropipettors Never: Let the plunger snap back
Immerse the barrel in fluid
Use a flame on the tip for sterilization

7. Using Micropipettors Two sizes: Yellow (smallest) 1-20 μL
Blue (largest) μL

8. Reading Micropipettors
Tens
Ones
Tenths

9. Reading Micropipettors
What’s the
reading for
this setting?
9.0 μL!!

10. Reading Micropipettors
Thousands
Hundreds
Tens

11. Reading Micropipettors
What’s the
reading?
100 μL

12. Reading Micropipettors
What’s the
reading?
570 μL

13. Directions for Use Set desired volume Put tip on barrel
Press plunger down to 1st stop position
Place tip into liquid
Slowly release plunger to draw up fluid into the tip

14. Directions for Use
6. Slide pipet tip out dragging it on the side wall to get rid of any excess droplets
7. Make sure there is no air space at tip end
8. Touch tip on inside of new tube and slowly depress plunger to first stop and then on to the second stop.