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Intratumoral Heterogeneity of MicroRNA Expression in Breast Cancer

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Presentation on theme: "Intratumoral Heterogeneity of MicroRNA Expression in Breast Cancer"— Presentation transcript:

1 Intratumoral Heterogeneity of MicroRNA Expression in Breast Cancer
Mithu Raychaudhuri, Tibor Schuster, Theresa Buchner, Katharina Malinowsky, Holger Bronger, Ulrike Schwarz-Boeger, Heinz Höfler, Stefanie Avril  The Journal of Molecular Diagnostics  Volume 14, Issue 4, Pages (July 2012) DOI: /j.jmoldx Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Reference genes (small RNAs U44 and U48 and miRNAs miR-16 and let-7a) were assessed by RT-qPCR to determine the best for use as normalization control. U48 showed the lowest level of intratumoral heterogeneity and intertumor variation, and was therefore used for RT-qPCR normalization. Box plots show the median (line within the box), 25th and 75th percentiles, with whiskers showing 1.5× the interquartile range; the small box sign shows the mean, and small cross signs show the maximum and minimum values. x axis values in italics and bold indicate cases containing lymph node metastases. *Invasive lobular cancers; (L) two largest primary tumors (7.9 and 8.0 cm diameter, respectively); †lymph node samples only. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 Intratumoral heterogeneity and variation among different patients in the relative expression of four candidate miRNAs (miR-10b, miR-210, miR-31, and miR-335) using U48 as a reference gene. Box plots show the median (line within the box), 25th and 75th percentiles, with whiskers showing 1.5× the interquartile range. The small box sign shows the mean, and small cross signs show the maximum and minimum values. *Invasive lobular cancers; (L) two largest primary tumors (7.9 and 8.0 cm diameter, respectively); †lymph node samples only. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

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