1. 1.4 Microscopy and Measurement
Microscopes
*Produce enlarged image of object
Magnification
*Increase an objects size
Resolution
*To show details clearly

2. Light Microscope (LM) http://www.biologycorner.com/microquiz/
Specimen must be thin enough to be transparent
Know the parts
The maximum magnification is about 400X
Power of magnification equals ocular lens times objective lens
Ocular lens are usually 10X
There are 3 objective lenses
*low power is 4X
*medium power is 10X
*high power is 40X

3. Electron Microscopes
Used to examine things smaller than cells like viruses
Uses a beam of electrons rather than light
Much more powerful than LM
There are 2 types. Neither are used to view live specimens.

4. 1. Transmission Electron Microscope (TEM)
Electrons flow through thinly sliced specimen, good to see internal details
Magnifies up to 200,000X

5. 2. Scanning Electron Microscope (SEM)
Specimen is not sliced but surface of specimen is sprayed
Magnifies up to 100,000X

6. Measurement Scientists use one single system of measurement
SI – International System of Measurements
7 fundamental base units

7. Measurements Base Quantity Name Abbreviation 1. length meter m
2. mass kilogram kg
3. time second s
4. electric current Ampere A
5. thermodynamic temp. Kelvin K
6. amount of substance mole mol
7. luminous intensity candela cd

8. prefixes abbreviation factor of base
giga G 1 billion
mega M 1 million
kilo k 1,000
hecto h
deka da 10
base base base
deci d 1/10
centi c 1/100
milli m 1/1000
micro u millionth
nano n billionth
pico p trillionth

9. Measurements kilo, hecta, deka, base, deci, centi, milli
Convert 12.3m into mm, dm, and km