1. Volume 118, Issue 1, Pages 101-107 (January 2000)
HCO3− secretion in the rat colonic crypt is closely linked to Cl− secretion 
John P. Geibel*,‡, Satish Singh‡,§, Vazhaikkurichi M. Rajendran§, Henry J. Binder‡,§ 
Gastroenterology 
Volume 118, Issue 1, Pages (January 2000)
DOI: /S (00)
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2. Fig. 1
Effect of several secretogogues on net bicarbonate movement in isolated, perfused colonic crypt. After completion of a control period (2), either 0.5 mmol/L DBcAMP (▨), 60 nmol/L VIP (■), or 100 nmol/L ACh (▩) was added to the bath solution. Positive numbers represent net absorption, negative ones net secretion.
Gastroenterology  , DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

3. Fig. 2
Bicarbonate movement in isolated, perfused colonic crypt. After completion of a control period (2), (A) 60 nmol/L VIP (▩) or (B) 0.5 mmol/L DBcAMP (▨) was added to the bath solution. The experiments indicated as 22 mmol/L HCO3− contained 22 mmol/L HCO3− with 5% CO2; those indicated as 0 mmol/L HEPES contained 32 mmol/L HEPES-replaced HCO3−. Positive numbers represent net absorption, negative ones net secretion. Numbers in parentheses represent numbers of crypts studied in each experiment.
Gastroenterology  , DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

4. Fig. 2
Bicarbonate movement in isolated, perfused colonic crypt. After completion of a control period (2), (A) 60 nmol/L VIP (▩) or (B) 0.5 mmol/L DBcAMP (▨) was added to the bath solution. The experiments indicated as 22 mmol/L HCO3− contained 22 mmol/L HCO3− with 5% CO2; those indicated as 0 mmol/L HEPES contained 32 mmol/L HEPES-replaced HCO3−. Positive numbers represent net absorption, negative ones net secretion. Numbers in parentheses represent numbers of crypts studied in each experiment.
Gastroenterology  , DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

5. Fig. 3
Effect of HCO3−/CO2 on net fluid movement in isolated, perfused colonic crypt. The experiments indicated as 22 mmol/L HCO3− contained 22 mmol/L HCO3− with 5% CO2; in those indicated as 0 mmol/L HCO3−, 32 mmol/L HEPES replaced 22 mmol/L HCO3−. In the top panel, 0.5 mmol/L DBcAMP (▨) was added to bath solution; in the bottom panel, 100 nmol/L VIP (■) was added to bath solution. 2, Control. Positive numbers represent net absorption, negative ones net secretion. Numbers in parentheses represent numbers of crypts studied in each experimental group.
Gastroenterology  , DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

6. Fig. 4
Role of lumen and bath Cl− on DBcAMP-induced net fluid and bicarbonate secretion in isolated, perfused colonic crypt. After completion of a control period (2) with a lumen solution containing mmol/L Cl−, 0.5 mmol/L DBcAMP (▨) was added to bath solution; Cl− was replaced by gluconate in both the lumen Cl− (■) and bath Cl− (▩) experiments. Positive numbers represent net absorption, negative ones net secretion. Numbers in parentheses represent numbers of crypts studied in each experiment.
Gastroenterology  , DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

7. Fig. 5
Effect of transport inhibitors on HCO3− and fluid movement. Fluid and HCO3− secretion was stimulated by bath 0.5 mmol/L DBcAMP (2). In the lumen DIDS experiment (▨), 100 μmol/L DIDS was added to lumen; in the bath bumetanide experiment (■), 100 nmol/L bumetanide was added to the bath solution; and in the lumen NPPB experiment (▩), 10 μmol/L NPPB was added to lumen solution. Positive numbers represent net absorption, negative numbers net secretion. Numbers in parentheses represent numbers of crypts studied in each experiment.
Gastroenterology  , DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions