1. Example of amplicon performance in our presented workflow.
Example of amplicon performance in our presented workflow. The bacterial HMP Mock Community “B” sample (BEI Resources) was processed using 16S V4-V5 (515F+926R [28]) and V6-V8 (B969F+BA1406R [27]) primer sets through our on-bench and bioinformatics workflow. Three replicates for each region were sequenced on three independent MiSeq runs and then normalized to 15,000 reads each during analysis. The mock sample contains equal amounts of rRNA gene copies of 20 species, one for each of the genera listed in the legend, except two species of Staphylococcus and three species of Streptococcus. Note that the individual variable regions have difficulty separating Escherichia from Shigella and that they are listed with interchangeable identities here. The output numbers (always 37 or 54) and identities of the OTUs were consistent between each replicate of a given variable region.
André M. Comeau et al. mSystems 2017; doi: /mSystems