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Efficiency of slush nitrogen vitrification of human oocytes vitrified with or without cumulus cells in relation to survival rate and meiotic spindle competence 

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Presentation on theme: "Efficiency of slush nitrogen vitrification of human oocytes vitrified with or without cumulus cells in relation to survival rate and meiotic spindle competence "— Presentation transcript:

1 Efficiency of slush nitrogen vitrification of human oocytes vitrified with or without cumulus cells in relation to survival rate and meiotic spindle competence  Maria Giulia Minasi, M.Sc., Gemma Fabozzi, M.Sc., Valentina Casciani, Ph.D., Susanna Ferrero, M.D., Katarzyna Litwicka, M.D., Ermanno Greco, M.D.  Fertility and Sterility  Volume 97, Issue 5, Pages (May 2012) DOI: /j.fertnstert Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Group A. (A–C) Oocyte with CCs during vitrification: before freezing (A), in preincubation solution (B), and in equilibration solution (C). (D–H) Oocyte during warming in the five solutions with decreasing sucrose concentrations. (I, J) Thawed oocyte after gentle decumulation (I) and during the PolScope analysis (J; white arrow indicates the MS). Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Group B. (A–C) Oocyte without CCs during vitrification: before freezing (A), in preincubation solution (B), and in equilibration solution (C). (D–H) Oocyte during warming in the five solutions with decreasing sucrose concentrations. (I) Thawed oocyte during the PolScope analysis (white arrow indicates the MS). Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions


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